IN VITRO ANTIBACTERIAL ACTIVITY OF PIPER BETEL LEAVES EXTRACT AGAINST STAPHYLOCOCCUS AUREUS AND CANDIDA ALBICANSAbstract
Endodontic irrigating solution used in root canal treatment in dental specialty is to eliminate root canal pathogens in necrotic pulp tissues. A microorganism like Staphylococcus aureus, Candida albicans, Enterococcus faecalis were commonly seen pathogens in necrotic pulp tissues. Commonly used root canal irrigating solutions to kill bacteria’s in the dental root canal are hydrogen peroxide (H2O2), chlorhexidine (CHX) and sodium hypochlorite (NaOCl). These root canal irrigating solutions has detrimental effects on the tissues underlying tooth (periradicular tissues) and affects post-operative healing. NaOCl has tissue toxicity and inhibits phagocytosis. Chlorhexidine inhibits protein synthesis in periodontal ligament cells. CHX and NaOCl exhibit an inflammatory effect in mitochondrial activity in human periodontal cells. Natural products are in great demand for their extensive biological properties and their bioactive molecules. Nontoxic Piper betel leaves extract obtained by methanolic extraction method of 500 mg/ml was tested against Staphylococcus aureus and Candida albicans which are commonly associated with root canal treatment and in failure cases using well agar diffusion method. The result shows piper betel leaves extract has antibacterial activity against these organisms.
G. Vinodh *, M. Kavitha and Pradeep
Department of conservative dentistry, Tamilnadu Govt dental college, Chennai, Tamil Nadu, India
29 October 2014
21 November 2014
29 November 2014
01 December 2014