IN-VITRO ANTIBACTERIAL ACTIVITY OF ACACIA ETBAICA AGAINST STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLIAbstract
This study was conducted to determine the in-vitro anti-microbial activity of Acacia etbaica, native plant to east African countries, against Staphylococcus aureus and Escherichia coli. To achieve this, the methanol extract of leaf of Acacia etbaica was tested for its in-vitro antibacterial activity against Staphylococcus aureus and Escherichia coli using agar disc diffusion method at two different concentrations (500 µg/disc and 1000 µg/disc). The minimum inhibitory concentration (MIC) of the plant crude extract was also determined using the microdilution method in 96-well plates. Acacia etbaica showed significant antibacterial activity with the mean zone of inhibition of 13.34 ± 1.04 mm and 11.13 ± 1.04 mm in diameter at a concentration of 1000µg of plant extract per disc against S. aureus and E. coli respectively. The MIC of the crude extracts of Acacia etbaica was determined to be 0.039 mg/ml and 0.313 mg/ml against S. aureus and E. coli respectively. The results suggest that the methanol extract of Acacia etbaica could be a rich source of antibacterial compounds. The results also indicate merit in the Acacia etbaica ethnomedicine use by the local communities.
B. Getachew *, S. Getachew, B. Mengiste and A. Mekuria
College of Veterinary Medicine, Mekelle University, Ethiopia
07 October 2015
26 November 2015
29 November 2015
30 December 2015