HPLC IDENTIFICATION OF CAFFEOYLQUINIC ACID DERIVATIVES OF CEPHALARIA GIGANTEA
AbstractSix phenolic acids -chlorogenic acid (1), caffeic acid (2), 4,5 di-O-caffeoylquinic acid (3), 3,5 di-O-caffeoylquinic acid (4), 3,4 di-O-caffeoylquinic acid (5) and 3,4,5-tri-O-caffeoylquinic acid (6) were identified by HPLC-UV-MS from the roots of Cephalaria gigantea (Dipsacaceae) growing in Georgia. The aim of the present research was a qualitative identification of phenolic constituents in an anticonvulsive enriched fraction from the roots of C. gigantea, for further quantitative standardization. The HPLC profile demonstrated a good resolution of phenolic acids of the active extract within 25 min of gradient mode (ACN 5% to 40%). On the basis of retention times, using the UV and MS spectra of the corresponding standards, compounds 4 and 5 were found to be majors in the analyzed fraction and were reported for the first time from C. gigantea roots. These experimental data could be a solid base for the future quantitative standardization of the anticonvulsive fraction for the prevention and treatment of psychosomatic forms of epilepsy.
Article Information
3
155-158
695
411
English
IJP
N. Tabatadze *, I. Tsomaia, A. Pichette and V. Mshvildadze
Faculty of Natural Sciences, Mathematics, Technology and Pharmacy, Ivane Javakhishvili Tbilisi State University, 26A. Politkovskayast, 0186 Tbilisi, Georgia.
nino_tabatadze@yahoo.com
27 November 2020
23 April 2021
29 April 2021
10.13040/IJPSR.0975-8232.IJP.8(4).155-58
30 April 2021
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