HPLC IDENTIFICATION OF CAFFEOYLQUINIC ACID DERIVATIVES OF CEPHALARIA GIGANTEAAbstract
Six phenolic acids -chlorogenic acid (1), caffeic acid (2), 4,5 di-O-caffeoylquinic acid (3), 3,5 di-O-caffeoylquinic acid (4), 3,4 di-O-caffeoylquinic acid (5) and 3,4,5-tri-O-caffeoylquinic acid (6) were identified by HPLC-UV-MS from the roots of Cephalaria gigantea (Dipsacaceae) growing in Georgia. The aim of the present research was a qualitative identification of phenolic constituents in an anticonvulsive enriched fraction from the roots of C. gigantea, for further quantitative standardization. The HPLC profile demonstrated a good resolution of phenolic acids of the active extract within 25 min of gradient mode (ACN 5% to 40%). On the basis of retention times, using the UV and MS spectra of the corresponding standards, compounds 4 and 5 were found to be majors in the analyzed fraction and were reported for the first time from C. gigantea roots. These experimental data could be a solid base for the future quantitative standardization of the anticonvulsive fraction for the prevention and treatment of psychosomatic forms of epilepsy.
N. Tabatadze *, I. Tsomaia, A. Pichette and V. Mshvildadze
Faculty of Natural Sciences, Mathematics, Technology and Pharmacy, Ivane Javakhishvili Tbilisi State University, 26A. Politkovskayast, 0186 Tbilisi, Georgia.
27 November 2020
23 April 2021
29 April 2021
30 April 2021