EVALUATION OF IN-VIVO & IN-VITRO ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC LEAF EXTRACT OF PIPER ATTENUATUM B. HAMAbstract
Inflammation is initiated as a healing process by the tissue in response to an injury by pathogens, irritants or cell damage. Piper attenuatum B. Ham is a rare piper species found in the tropical, sub-tropical region and mainly found in the southern part of India. A plant has constituents like alkaloids, amides, glycosides, tannins etc., which are responsible for its therapeutic efficacy. This research focuses on the use of leaves of Piper attenuatum B. Ham as anti-inflammatory agents. The phytochemical and analytical evaluation was done for ethanolic leaf extract. Various part of the plant is used for treating fever, malaria, diuretic, and cancer. Piperine is a major alkaloid found in almost all piper species & responsible for anti-inflammatory activity. In the present study, ethanolic leaf extract of Piper attenuatum B. Ham at doses 200mg/kg and 400mg/kg showed significant in-vivo anti-inflammatory activity in carrageenan-induced paw edema in rats and in-vitro anti-inflammatory activity by protein denaturation method for dose 500, 400, 300, 200 & 100 μg/mL.
Gaurav Kumar *, Gurpreet Singh, Nitin Jumnani, Shafeequllah and Nupur Ratnawat
Department of Pharmacology, Lords International College of Pharmacy, Lords University, Chikani Alwar, Rajasthan, India.
08 March 2022
26 April 2022
28 April 2022
30 April 2022