EVALUATION OF IN-VITRO ANTI-INFLAMMATORY ACTIVITY OF HYDROETHANOLIC EXTRACT OF DELOSPERMA COOPERI PLANT

Objective: The main objective of this study is to evaluate the anti-inflammatory activity of hydroethanolic extract of the entire plant except for the roots and flowers of Delosperma cooperi (Family: Aizoaceae), also known as hard ice-plant by in -vitro protein denaturation method. However, the anti-inflammatory effects of D. cooperi has not been studied. Method: The D. cooperi were extracted by the soxhlation method by using the hydroethanolic solution (60; 40) ratio. Then the extract was evaporated to dryness in order to yield dried crude extract of D. cooperi. The preliminary studies of phytochemicals were done for the confirmation of the presence of active constituents for the effective pharmacological activity. The anti-inflammatory activity was analyzed by using chicken’s egg albumin denaturation method (in-vitro). The hydroethanolic extract was found to have a high ability to inhibit protein denaturation that was evaluated by using a UV spectrophotometer. Result: The extracts of D. cooperi exhibited concentration-dependent protein (Albumin) denaturation inhibition. The effect of the test sample was found to be good when compared with the diclofenac (standard). Conclusion: From the obtained results, it can be concluded that D. cooperi possessed a good anti-inflammatory activity against protein denaturation of egg albumin. The anti-inflammatory activity may be due to the presence of alkaloid as well as flavonoids. The anti-inflammatory activity of the extract can be established as a potential source for the development of new treatment against inflammation.