EVALUATION OF ANTIDIABETIC ACTIVITY OF HETEROPHRAGMA QUADRILOCULARE (ROXB.) K. SCHUM. LEAVESAbstract
Heterophragma quadriloculare (Roxb.) K. Schum. (HQ) belongs to Bignoneace family and known as Warras. Usage of HQ plant in diabetes has been claimed traditionally, but scientific documentation is not yet available. So, it was our interest to develop a scientific database for HQ leaves. HQ leaves were studied for its traditional claim of anti-diabetic activity by oral glucose tolerance test (OGTT) model using normal rats. All possible phytoconstituents were extracted into a selected range of solvents based on its polarity, i.e., petroleum ether, chloroform, methanol, water. The extracts were screened for OGTT at a dose level of 200 and 400 mg/kg per oral. In OGTT study through all extracts found to reduce blood glucose level, petroleum ether extract and chloroform extract showed dose-dependent activity. These results provided the clue for the presence of anti-diabetic constituents in petroleum ether extract. Thus petroleum ether extract (200 mg/kg), an unsaponifiable fraction of petroleum ether extract (100 mg/kg) and a saponifiable fraction of petroleum ether extract (100 mg/kg) of HQ were studied in rats by STZ-NAD induced type-II diabetic model up to 21 to find out the most potent anti-diabetic fraction. Blood glucose and lipid profile were recorded on 0th and 21st day. Oral administration of PEHQ and UPEHQ resulted in significant weight gain, reduction of blood glucose, serum cholesterol, serum VLDL and triglycerides in diabetic rats as compared to diabetic control rats. The unsaponifiable fraction of petroleum ether extract at the dose of 100 mg/kg showed significant antidiabetic activity. The present study thus justifies the traditional claim of Heterophragma quadriloculare (Roxb.) K. Schum. for diabetes. The unsaponifiable fraction of petroleum ether extract of HQ needs further attention for separation and identification of biologically active compounds that are unidentified yet.
B. H. Satani*, V. S. Surana, S. A. Shah and S. H. Mishra
Maliba Pharmacy College, Uka Tarsadia University, Bardoli, Gujarat, India.
07 June 2016
21 August 2017
17 September 2017
01 November 2017