EFFECT OF DESMOSTACHYA BIPINNATA EXTRACTS ON CASTOR OIL AND MAGNESIUM SULPHATE INDUCED DIARRHEA IN WISTAR RATSAbstract
Desmostachya bipinnata (L.) Stapf [Gramineae (Poaceae)] is used in Indian traditional medicines system for treatment of various diseases such as asthma, kidney stone, diarrhea, wound healing, etc. These traditional claims require authentication by performing animal testing. This study aims to confirm the antidiarrheal activity of D. bipinnata in Wistar rats. Underground parts of D. bipinnata was extracted in alcohol, water, a hydroalcoholic solution, chloroform, and ethyl acetate. Acute toxicity test was performed in albino mice and antidiarrheal activity was estimated in Wistar rats. Castor oil and magnesium sulfate-induced diarrhea in Wistar rats were treated with single oral administration of prepared D. bipinnata extracts (500 mg/kg) and antidiarrheal drug loperamide (3 mg/kg). Diarrhoeal droppings were observed for 6 h in all treated animals. One way ANOVA followed by Dunnet’s ‘t’ test was used to identify significant differences among obtained results. Extracts of D. bipinnata showed neither mortality nor any toxic effect in albino mice up to the dose of 5 g/kg in 48 h to 14 days. The alcohol extract of D. bipinnata was 61.54% (P<0.01) effective in reducing feces in castor oil induced-diarrheal rats and 64.52% (P<0.01) feces reduction in magnesium sulphate induced-diarrheal rats. Extracts of D. bipinnata were found effective in reducing diarrhea in Wistar rats Therefore, it is anticipated that D. bipinnata contains pharmacologically active substances responsible for antidiarrheal activity.
A. Singh *, V. A. Saharan, V. Ram and A. Bhandari
Faculty of Pharmaceutical Sciences, Jodhpur National University, Boranada, Jodhpur, Rajasthan, India.
13 September 2014
21 October 2014
29 October 2014
01 November 2014