CULTURING OF MELASSEZIA FURFUR AND ITS GROWTH INHIBITING ACTIVITY OF GARCINIA INDICAAbstract
Dandruff is a common disorder affecting the scalp. Malassezia furfur, the lipophilic dimorphic yeast-like fungus is the main causative agent of dandruff. In recent years plant-based products are widely used as the therapeutic weapon to cure human disorders. The plant Garcinia indica (Kokam) belonging to family Clusiaceae native to India is one of such plants which has shown many therapeutic uses. One part of the present study shows that the optimum conditions for the growth of Malassezia furfur were on sabouraud’s dextrose liquid medium supplemented with corn oil at 30 °C with pH ranging between 8 and 9 and the other part emphasizes on the screening of M. furfur against fractions separated from fruit rinds of G. indica. Out of three screened fractions (Methanolic extract, water fraction and ethyl acetate fraction), ethyl acetate fraction showed a good zone of inhibition 18.2 ± 0.3 mm and 22.6 ± 1.22 mm at 75 and 100% concentration respectively.
A. Sahasrabudhe* and M. Phanse
Research Laboratory, Department of Botany, D.S.P.M’s K.V. Pendharkar College of Arts, Science and Commerce, Dombivli (E), Thane, Maharashtra, India
17 June 2015
15 July 2015
14 August 2015
31 August 2015