CHANGES IN TESTIS PROTEIN AND METABOLIC ENZYME ACTIVITIES IN RATS INDUCED BY EPICHLOROHYDRINAbstract
In the present study, an attempt was made to evaluate the changes in testis protein and metabolic enzyme activities induced by epichlorohydrin (ECH), twenty-four albino male rats were divided randomly into four groups of six rats each. One group was left untreated as controls, and the other three groups were administered, respectively, for twelve consecutive weeks, (2) 50 mg of ECH /kg/b.wt, (3) 75 mg of ECH /kg/b.wt and (4) 100 mg of ECH /kg/b.wt. In comparison with the control group, the doses of ECH (75 mg/kg/b.wt and 100 mg/kg/b.wt) resulted in lower protein levels and higher lactate dehydrogenase (LDH) activities in the testis of male rats than in control rats. The activity of gamma-glutamyl transpeptidase (γ-GT) decreased significantly at the doses of ECH (75 mg/kg/b.wt and 100 mg/kg/ b.wt) group than in the ECH (50 mg/kg/b.wt) and control group. However, a significant reduction in the activities of Na+/K+– , Mg2+-and Ca2+-ATPases in the (75 mg/kg/b.wt and 100 mg/kg/b.wt) ECH treated animals than control rats. Therefore, these changes in testis protein and spermatogenesis-dependent enzymes undoubtedly affect the physiological functions of the testis, which may thereby cause low sperm motility.
K. Muthu, S. Thirukannan, K. Karthikeyan, P. Ilansuriyan and M. Marimuthu *
Research and Development Division, AquAgri Processing Private Limited, Manamadurai, Sivaganga, Tamil Nadu, India.
30 April 2015
24 June 2015
28 June 2015
30 June 2015