BIOLOGICAL EVALUATION OF IN VIVO DIURETIC AND ANTIUROLITHIATIC ACTIVITIES OF LEAF EXTRACTS OF MELOCHIA CORCHORIFOLIAAbstract
The present study was investigated to establish the diuretic and antiurolithiatic activities of chloroform and ethanolic leaf extract of Melochia corchorifolia. The chloroform and ethanolic leaf extract were administered to experimental rats orally at a dose of 200 mg/ kg and 400 mg/kg. Furosemide (5 mg/kg) was used as reference standard for diuretic activity. The parameters measured for diuretic activity was total urine volume; urine electrolyte concentrations such as sodium, potassium, chloride, and bicarbonates. In the in-vitro antiurolithiatic activity, Calcium oxalate crystallization was induced by the addition of 0.01M sodium oxalate solutions in synthetic urine. The effect of chloroform and ethanolic leaf extract of Melochia corchorifolia (100, 200 and 500 μg/ml) was studied by time course measurement of absorbance at 620 nm for ten minutes using a spectrophotometer. Both the extracts show good in-vitro antiurolithiatic activity. In the in-vivo antiurolithiatic activity, urolithiasis was induced in male rats by administering ethylene glycol (0.75% v/v) in drinking water for 28 days, and the parameters such as oxalate, calcium, and phosphate were estimated in urine. Serum creatinine, calcium, and uric acid were also estimated. Treatment with the leaf extract of Melochia corchorifolia restored all biochemical, urinary parameters. The results obtained justified the importance of the leaf extract of Melochia corchorifolia as a diuretic and antiurolithiatic agent.
M. N. Palaksha *, K. Ravishankar and G. V. Sastry
Department of Pharmacy, Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, India.
31 March 2017
23 May 2017
30 May 2017
01 July 2017