BIOLOGICAL ACTIVITIES OF EXTRACTS AND SEASONAL VARIATION IN ESSENTIAL OIL COMPOSITION FROM MYRCIARIA GLAZIOVIANA (MYRTACEAE)Abstract
Extracts from leaves, stems and flower buds of Myrciaria glazioviana were analyzed for their antioxidant and anticholinesterase activity. The methanolic extracts showed the best results in reducing power (623.52 – 806.15 mg AA/g of extract), DPPH radical scavenging (IC50 = 9.77 – 14.06 µg/mL) and lipid peroxidation inhibition activity (21.30 – 66.80%). These data were correlated to the total phenolic content (270.19 – 351.01 mg GA/g of extract). The dichloromethane extract of leaves exhibited a higher anti-cholinesterase potential (IC50 = 249.25 µg/mL). Furthermore, the chemical composition of the essential oils from leaves collected in all seasons of the year was analyzed by GC-MS and GC-FID. Only sesquiterpenes were identified in all essential oils, and non-functionalized sesquiterpenes comprised the major fractions (from 66.8 to 79.3%). An increase in non-functionalized compounds was observed in the warmer seasons. Germacrene B was the major compound in all seasons (from 26.8 to 30.1%). In winter and autumn, high contents of germacrene D (10.2 and 10.0%, respectively) and α-cadinol (12.1 and 12.0%, respectively) were identified; germacrene D (14.0%) was among the major compounds in summer and β-elemene (11.6%), in spring.
G. A. Zatelli, P. Zimath, A. Tenfen, D. R. Scharf, E. L. Simionatto, M. D. Alberton and M. Falkenberg *
Programa de Pós-graduação em Farmácia, Universidade Federal de Santa Catarina, Rua Delfino Conti s/n, Florianópolis, SC, Brasil.
21 November 2016
19 December 2016
26 December 2016
01 January 2017