ASSESSMENT OF ANTIOXIDANT, ANTIBACTERIAL AND ANTHELMINTIC ACTIVITIES OF ETHANOL EXTRACT OF LEAVES OF CROTALARIA PALLIDA (AITON)Abstract
The ethanol leaves extract of Crotalaria pallida (Aiton) was evaluated to investigate the Phytochemical nature and some selected pharmacological activities. The plant extract indicated the presence of combined reducing sugar, tannins, flavonoids, glycosides, alkaloids and steroids. The extract showed free radical scavenging activity with an IC50 value of 37.60 µg/ml, and standard ascorbic acid showed IC50 value of 16.95µg/ml. The total phenolics content (TPC) was found to be 14 ± 0.015 mg GAE/g of dried plant extract. Total flavonoids content was found to be 20.42 ± 0.001 mg QE / 100 g of dry extract. The extract showed mild antibacterial activity against the bacterial strains Vibrio cholera, Shigella flexneri, Shigella dysenteriae, in comparison with standard drug kanamycin (30 µg/disc). In the anthelmintic test, the extract showed a dose-dependent decrease in paralysis time and death time of worm Paramphistomum cervi (trematoda) when compared with standard albendazole. These results espouse the traditional use of the leaves of this plant.
M. H. Alam *, G. M. M. Murshid, B. Bokshi, S. A. Suma, S. I. Sathi and A. Rahman
Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh.
10 February 2014
18 May 2014
28 June 2014
01 July 2014