ANTISECRETORY AND ANTIOXIDANT ACTIVITIES OF THE LEAVES AQUEOUS EXTRACT OF BOCSIA ANGUSTIFOLIA (CAPPARACEAE) ON GASTRIC ULCERS IN WISTAR RATSAbstract
The aim of this study was to evaluate antisecretory and antioxidant activities of the leaves aqueous extract of Boscia angustifolia (LAEBA) on gastric ulcers induced in male rats. LAEBA at the doses of 125, 250, and 500 mg/kg were tested on three models of gastric ulcers, namely: pylorus ligation, pylorus ligation + histamine, and pylorus ligation + carbamylcholine. Some parameters of oxidative stress (malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)) were evaluated in stomach’s homogenates. The qualitative phytochemical screening of LAEBA was carried out. Antisecretory screening showed that LAEBA at 500 mg/kg significantly decreased (p<0.01) gastric acidity (8.06 mEq/l) compared to the negative control (14.07 mEq/l), with a significant reduction (p<0.001) of gastric volume (1.30 ml) compared to the negative control (5.12 ml), as well as a reduction of pepsin activity (64.64%). These variations were similar to pylorus ligation + histamine and pylorus ligation + carbamylcholine models. LAEBA at all the doses induced a significant reduction (p< 0.05) of ulcerated surface correlated with an increase of mucus secretion (16.89 to 187.50%) compared to the negative control. It also induced a significant reduction of MDA level and a significant increase in catalase and GSH. LAEBA would protect the gastric mucous membrane by a mechanism which would involve the cholinergic and histaminic ways, the reinforcement of mucus secretion and antioxidant status, via the presence of flavonoids, saponins and tannins in LAEBA.
A. P. Amang *, J. Walantini, G. L. O. Ndji, G. T. Siwe, L. V. Vandi and P. V. Tan
Department of Biological Sciences, Faculty of Science, University of Maroua, Maroua, Cameroon.
21 July 2020
27 September 2020
29 September 2020
31 December 2020