ANTIOXIDANT ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF FRACTIONATED LEAF EXTRACTS OF CATHARANTHUS ROSEUSAbstract
Antioxidant effectiveness of indigenous medicinal plant C. roseus leaves extracts and fractions with solvents of different polarity (ethanol, methanol, acetone, hexane, butanol, and water) were assessed for DPPH radical scavenging activity. The C. roseus extracts and fractions contained appreciable levels of the antioxidant activities of leaves of C. roseus at various concentrations. The leaves of C. roseus showed good antioxidant activity (81.70%). The phytochemical analysis of the C. roseus shows the presence of alkaloids, terpenoids, steroids, flavonoids, and other plant secondary metabolites. GC-MS result shows that the important secondary metabolites and their derivatives present in C. roseus leaves extract. The results of the present comprehensive analysis demonstrated that C. roseus extracts and fractions are a viable source of natural antioxidants and might be exploited for functional foods and nutraceutical applications.
S. Patharajan* and S. Bala Abirami
Department of Biotechnology, PRIST University, Thanjavur, Tamil Nadu, India.
14 November 2013
13 January 2014
26 January 2014
01 February 2014