ANTIBACTERIAL ACTIVITY OF THE HYDRO-ETHANOLIC TRUNK BARK EXTRACT OF STEREOSPERMUM KUNTHIANIUM CHAM. TRUNK (BIGNONIACEAE) AND ITS FRACTIONSAbstract
Stereospermum kunthianum Cham. is a small, to medium-sized tree, sometimes only a small shrub. Bark grey, smooth, sometimes flaking, revealing pale under bark. In Senegal this plant is used in traditional medicine against gastritis, asthenia, respiratory diseases but also as a wound healing. The present study was taken up to research an anti-bacterial activity in the trunk bark hydro-ethanolic extract and its fractions obtained by using different solvents. Extract and fractions were tested on clinical strains and reference strains of four bacteria: Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis. The methods of diffusion in agar medium and micro-dilution in liquid medium were used. The results showed that on clinical strains, the lowest Minimum Inhibitory Concentration: MIC (0.156 mg/ml) was observed with the hydro-ethanolic extract and its aqueous fraction on Pseudomonas aeruginosa. With regard to the reference strains, the best activity was noted with the aqueous fraction with a MIC of 2.5 mg/ml on the Pseudomonas aeruginosa strain ATCC 27853. However, the standard antibiotics used are more effective than the extract and its fractions.
A. Sarr *, A. Dieng, S. I. M.Dieng, K. Sonko, D. Diatara, A. D. Fall and E. Bassene
Laboratory of Pharmacognosy and Botany, FMPO, UCAD, Dakar, B.P: 5005 Dakar Fann, Senegal.
29 January 2021
28 March 2021
30 March 2021
31 March 2021