ANTIBACTERIAL ACTIVITY OF GARLIC EXTRACTS AGAINST GRAM-NEGATIVE AND GRAM-POSITIVE BACTERIAAbstract
Garlic is one of the earliest well known medicinal plants, which used to cure different disease in a human being. It used to fight infections, prevent cancer, antidiabetic, hepatoprotective, anthelmintic, anti- antioxidant, and wound healing. Based on the above important of garlic, this study was conducted to investigate the antibacterial activity of garlic extracts (ethanol, acetone and aqueous extracts) against bacterial strains. The antibacterial activity of garlic was tested against gram-positive Bacillus subtitles and gram-negative E. coli bacterial strains by using agar well diffusion method. The results reveal that garlic extract shows inhibitory activity against gram-positive bacteria Bacillus subtilis and gram-negative E. coli bacterial strains. The maximum zone of inhibitions observed against Bacillus subtilis with the combination (32 mm), aqueous (30 mm), ethanol (26 mm), and acetone (22 mm) extracts, respectively. Minimum inhibition zone was observed in all extracts against Gram-negative Bacteria (E. coli), i.e. combination (21 mm), aqueous (16 mm), ethanol (14 mm), and acetone (12 mm). The results showed that B. subtilus is more susceptible to garlic extracts as compared to E. coli.
T. Derbe *, B. K. Mariam and S. Badasa
Department of Chemistry, Adigrat University, Adigrat, Ethiopia.
13 September 2014
21 October 2014
29 October 2014
01 November 2014