ANALGESIC AND ANTI-INFLAMMATORY ACTIVITIES OF VITEX NEGUNDO (LEAVES) EXTRACTAbstract
Aims: Analgesic and anti-inflammatory effects of Vitex negundo leaves extract (VNE) were evaluated in the animal models. The VNE was evaluated for the phytochemical analysis. Settings and Design: Factorial design was used for the experimental models. Methods and Material: The analgesic effects at graded doses of VNE (40-320 mg/kg, p.o.) were evaluated in mice against acetic acid-induced writhing (chemically induced pain) and hot-plate method (thermally induced pain). The analgesia produced by VNE was compared with the standard analgesics diclofenac sodium (DIS, 5 mg/kg, p.o.) and pentazocine (PTZ, 5 mg/kg, p.o.). Acute anti-inflammatory activity of VNE was also analyzed using carrageenan-induced rat paw edema model at the doses 40, 80 and 160 mg/kg i.p., using diclofenac sodium (5 mg/kg, i.p.) as standard. Statistical Analysis Used: ANOVA was applied followed my Tukey’s post hoc test for comparison between the group’s Results: In comparison to control group VNE showed highly significant, dose-dependent analgesic activity against chemically as well as thermally induced pain models (P<0.05). In comparison to control, VNE at the employed doses produced marked acute anti-inflammatory activity in rats (P<0.05). Conclusion: The results suggest that the aqueous extract of Vitex negundo has significant analgesic and anti-inflammatory potential as reflected by the parameters investigated. Further investigations are however necessary to explore mechanism(s) of action involved in these pharmacological activities.
P. S. Mishra*, S. Vyas, R. P. Agrawal, P. Phadnis and A. Tiwari
Department of Pharmacology, M.G.M. Medical College, AB Road, Indore, Madhya Pradesh, India.
17 March 2014
13 April 2014
30 April 2014
01 May 2014