Posted by admin on Mar 31, 2019 in |
Piplartine is an amide obtained from Piper tuberculatum, which is promising in antiparasitic tests. Therefore, it has been used as the basis for the synthesis of new molecules. This study was designed to analyze the immunomodulatory effect of piplartine (A) and its derivative (B), in-vitro, on macrophages infected with Leihmania amazonensis, as well as the production of hydrogen peroxide (H2O2), nitric oxide (NO) and lipid bodies. Were evaluated by staining with giemsa and counting the cells under optical microscopy; labeling with tetramethyl benzidine and peroxidase type II; Greiss reagent and oil red, respectively, with amides A and B concentrations ranging from 0.0 to 64 μg/mL. Modulation of the immune system was observed. There was a decrease in the infection index at the highest concentration (64 μg/mL) for A and B in infected macrophages. Amide A (4 μg/mL) reduced the H2O2 production of infected macrophages by 99.3% compared to control. Cultures treated with A and B increases the NO production at 16 μg/mL and 0.25 μg/mL, respectively. The production of...
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Posted by admin on Mar 31, 2019 in |
Ajuga chamaepitys (L.) Schreb, well-known as Camaepitiumor Ground Pine, is an annual herb typical of the Mediterranean area accounting several uses in the traditional medicine. In this study, the methanol extract of Ajuga chamaepitys (L.) Schreb was analyzed using Gas Chromatography-Mass Spectrometry (GC-MS), while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology Library. The study revealed the presence of fifteen phytocomponents. The most abundant compounds in the methanol extract were eucalyptol, thujone, endoborneol, davana ether, and octacosane. The results indicated that polarity of the solvents used in extraction influenced the relative abundance and type of compounds extracted. The various compounds identified are known to have varying bioactivities such as being hemorrhoids, stomachache, jaundice, and inflammatory diseases....
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Posted by admin on Mar 31, 2019 in |
Cancer is the one of the leading cause of death worldwide. Even though many treatments exists in treating and controlling the progression of the deadly disease cancer, yet important inadequacy for development remain. Several undesired side effects occur when cancer is treated using chemotherapy. Therapies of Mother Earth, that utilizes bioactive products derived from a plant in treating cancer, may lessen the side effects. North-East India is recognized as a rich ethnomedicinal asset. It contributes to the abundant reservoir of plant diversity and is recognized as one of the ‘biodiversity hotspots’ of the world reinforcing around 50% of the country’s biodiversity. There has been an increasing frequency of occurrence of cancer in developing countries like India, Nepal, and Pakistan which has serious implications on the morbidity and mortality in millions of people. Most of the Himalayan plants that have been reported are biologically active against different cancer types. The present statistics of cancer chemotherapy is bleak which is why there is an urgent need to develop and discover potent...
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Posted by admin on Feb 28, 2019 in |
Malaria is a significant public health problem in the world, and the rising problem of drug resistance and the availability of a limited number of effective anti-malarial drugs underline how essential it is to find new anti-malarial agents. This study was undertaken to evaluate the in-vivo antimalarial activities of an extract of Hagenia abyssinica that is used traditionally to treat malaria in Ethiopia. The rodent malaria Plasmodium berghei was used to infect healthy 7-week-old Swiss albino mice weighing 24-28 gm. Each of the extracts (100 mg/kg, 200 mg/kg, and 400 mg/kg) was administered to different groups of mice. The parameters of parasitemia, survival time, body weight, and packed cell volume were measured using repository test and Peter’s test. The extract significantly inhibited parasitemia and increased survival time in infected mice in a dose-dependent manner. The extract prevented loss of weight, packed cell volume. This study concluded that the extract has a promising anti-malarial activity which supports the in-vitro anti-plasmodial activity and the traditional use of the plant for treatment...
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Posted by admin on Feb 28, 2019 in |
Withania somnifera is a traditional indigenous plant having numerous medicinal values. With renewed interest, the present study was undertaken to determine its new potential therapeutic benefit. We aimed to evaluate the effect of methanolic extract of Withania somnifera leaves in L-methionine induced hyperhomocysteinemia in rats. Wistar rats (250-300 gm) were divided into 5 groups (n=6/group): Group I= Normal control (vehicle, 1% CMC); Group II= Hyperhomocysteinemic rats (L-methionine, 1 gm/kg, p.o.); Group III= Methanolic extract of Withania somnifera (200 mg/kg, p.o.); Group IV= Methanolic extract of Withania somnifera (400 mg/kg, p.o.); Group V= Folic acid (1 gm/kg, p.o.). All the rats except normal control group were treated with L-methionine for the induction of hyperhomocysteinemia. Animals of Group III-V were subjected to the respective treatment for 30 days. On completion of the study, serum homocysteine, cholesterol, triglyceride, LDL, VLDL, and HDL level were determined. Administration of L-methionine significantly increased the serum homocysteine, cholesterol, triglyceride, LDL, VLDL levels while decreased HDL level compared to normal control. Methanolic extract of Withania somnifera (200...
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