Posted by admin on Jan 11, 2018 in |
In the present study, an attempt was made to formulate ethosomal gel containing leaf extract of Mangifera indica, and was evaluated for antibacterial activity against gram-positive and gram-negative organisms. Different formulations of ethosomes using lecithin, cholesterol, and ethanol were prepared using Mangifera indica herbal leaf extracts. Carbopol 940 was used to prepare ethosomal gel. The entrapment efficiency of ethosomes was 65.1% – 96.54% and the average vesicle size was 920 nm. Three formulations of different doses (100, 200 and 300 mg) were selected based on entrapment efficiency and drug release. Prepared gels were then evaluated for physicochemical characteristics and drug content. The antibacterial activity was done using agar well diffusion method for the zone of inhibition. The pH of the gel formulations was found to be in the range of 5.4 – 6.2 and viscosities between 2250 – 2399 centipoises. The drug content of gels ranged between 74.67 – 82.31%. The zone of inhibition was measured as 9 mm for the dose of 200 mg formulation for gram-positive bacteria,...
Read More
Posted by admin on Jan 11, 2018 in |
Identification of natural marine bioactive molecules and their therapeutic potential is valuable considering that there are reduced chances of undesirable side effects. This study was designed to isolate and purify the polysaccharides from E. intestinalis and evaluate its anti-cancer effect on human hepatocarcinoma cell lines (Hep3B). Polysaccharides from Enteromorpha intestinalis were isolated and purified. Preliminary characterization was done using UV-Vis spectrum and FTIR analysis. Total sulfate content of the polysaccharides was determined. MTT assay, AO / EB and DAPI staining were done to determine the cell growth pattern and nuclear changes effected by polysaccharides on Hep3B cells. Effect of polysaccharides on mitochondrial membrane potential and ROS production on Hep3B cells were evaluated using Rhodamine 123 and DCHF-DA staining. Results showed that polysaccharides from E. intestinalis were rich in sulfate groups (sPS). Cell viability, assessed in-vitro by MTT assay showed that the number of viable cells was less than 50% when exposed to concentrations exceeding 1 mg/ml of sPS. Cell viability and nuclear integrity assays depicted cells with compromised membrane...
Read More
Posted by admin on Jan 11, 2018 in |
Molecular beacons have shown their topnotch potential in a variety of basic research, biomedical detection and clinical diagnosis. Their excellent selectivity, sensitivity, detection without separation have made them widely accepted tool for nucleic acid analysis. MBs present high-throughput screening of SNPs. The use of SNPs in the detection of genetic disorders is facilitated by the recent discovery of more than 4 million SNPs in the human genome. The objective of this study was to explore the association of rs699 SNP with essential hypertension in Punjabi Population. MB based biosensor developed by Verma et al., in 2016 was chosen for analysis. These hypertensive subjects tested positive against hypertensive biosensor (against allele C) were associated with essential hypertension. C4072T or rs699 polymorphism was genotyped in 50 hypertensive and 50 normotensive subjects. The p-value for the C allele in hypertensive patients was 0.71, which concludes susceptibility to hypertension in this population when the CC genotype is present. The implementation was cross-validated by applying the same samples to Normotensive Biosensor (against T allele)....
Read More
Posted by admin on Jan 11, 2018 in |
Bacillus cereus is a gram-positive, rod-shaped, motile, beta hemolytic bacteria. These bacteria cause foodborne illness, severe nausea, vomiting, diarrhea, and keratitis. Foodborne illness caused by Bacillus cereus occurs because of the bacterial endospores survival in improperly cooked food. The bacteria produce enterotoxins having high resistance against heat and acids, and they cause the diarrheal and emetic syndrome. The diarrhetic syndromes observed in patients are thought to stem from the three toxins: hemolysin BL (Hbl), non-hemolytic enterotoxin (Nhe) and cytotoxin K (CytK). These enterotoxins are all produced in the small intestine of the host, thus thwarting digestion by endogenous host enzymes. The effect is the loss of cellular membrane potential and eventually cell death. Antibiotics that are used for the treatment of infectious disease have suffered a major setback due to the development of drug resistance by the pathogenic target organism. Therefore, in the current study, the riboswitches are explored as an alternative potential drug target. A riboswitch of Bacillus cereus bacteria has been identified as a drug target, and...
Read More
Posted by admin on Jan 11, 2018 in |
The study aims to standardize a vaginal herbal formulation consisting of stem barks of Ficus glomerata Roxb. and Symplocos racemosa Roxb. Since they are used as Ayurvedic medicine and described in ancient text Charak Samhita as astringent, anti-inflammatory and treatment of vaginitis/leucorrhoea. This new herbal formulation is likely to exhibit significant antimicrobial action due to the presence of phenolic and flavonoid compounds. The extractive value was 1.64 and 1.70% w/w for aqueous and alcoholic extract. Total Phenol Content was 225.67 and 220.00 µg Gallic acid equivalent/mg for aqueous and alcoholic extract. Fifteen types of mucoadhesive tablets weighing 550, 700 or 900 mg each were prepared using combinations of research drug, Carbopol and Hydroxy-Propyl-Methyl Cellulose as polymers, talc as a glidant, magnesium stearate as a lubricant and Micro-Crystalline Cellulose and Lactose monohydrate as diluents. Similarly, five types of effervescent mucoadhesive tablets using sodium bicarbonate and citric acid in 3:1 ratio were prepared. Apart from weight, thickness, hardness, and friability of each group, the surface pH, swelling index and muco-adhesive force...
Read More