ANTIBACTERIAL ACTIVITY OF METHANOLIC EXTRACT OF MANGIFERA INDICA (BARK) AND OSYRIS LANCEOLATA (LEAVES) FROM WESTERN REGION OF NEPAL
AbstractNature has been a source of medicinal agents since times immemorial. The importance of herbs in the management of human ailments cannot be overemphasized. Different extracts from traditional medicinal plants have been tested to identify the source of the therapeutic effects. As a result, some natural products have been approved as new antibacterial drugs. The objective of this research is to find out the potent antibacterial agents from the selected medicinal plants: Mangifera indica and Osyris lanceolata of Rupandehi, and Palpa districts of Nepal. The Bark and Leaves of the plants Mangifera indica and Osyris lanceolata respectively were subjected to extraction by maceration process using solvent methanol. Screening of antibacterial activity was done by disc diffusion method against the microorganisms Staphylococcus aureus and Escherichia coli at three different concentrations (1 mg/disc, 2 mg/disc, and 4 mg/disc). Both plants showed potent antibacterial activity. Minimum inhibitory concentration (MIC) assay was determined for the two extracts against these bacterias. The concentration 4 mg/disc of M. indica showed maximum activity against both E. coli and S. aureus, i.e., 11.16 ± 0.28 and 11.33 ± 0.29 respectively. Although both plants have exhibited best results against gram-positive as well as gram-negative bacteria, the crude extract of these plants further needs to be purified through antibacterial activity guided fractionation to isolate and identify the compounds responsible for the antibacterial activity. So, this study provides the platform to carry out further researches to give light upon specific compounds which are responsible for their antibacterial activities.
Article Information
3
200-207
583
738
English
IJP
P. S. Bhandari, R. Bhandari, B. K. Sah, S. Gyawali, M. Bhusal, S. Shrestha and S. Shakya *
Market Planning Department, Everest Pharmaceuticals Pvt. Ltd, Tinkune, Koteshwor, Nepal.
ssujyoti@gmail.com
03 March 2017
17 May 2017
30 May 2017
10.13040/IJPSR.0975-8232.IJP.4(6).200-07
01 June 2017