Posted by admin on Jan 11, 2018 in |
Objective: To evaluate the chemical composition, antioxidant and antiproliferative activities of Hyptis pectinata aerial part aqueous extract (HYP). Method: Chemical profiling using high-performance liquid chromatography with UV diode array detector (HPLC-UV-DAD), in-vitro antioxidant and anti-proliferative studies were done. Antioxidant and antiradical activities of HYP was investigated using 3-(4,5-dimethylimidazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and DPPH radical scavenging assay. Result: The present study revealed several metabolites. HPLC analysis gave caffeic acid (13.92%), rutin (7.89%) and ferulic acid (5.44%) as some of the bioactive constituents. HYP showed antioxidant activity in both the DPPH and MTT assay. HYP exhibited antioxidant activity against DPPH and MTT from the first dilution at 10 mg/ml up to the ninth dilution at 0.08 mg/ml in a 2-fold dilution signifying the presence of antioxidant compounds at those concentrations. At 1 mg/ml to 32 mg/ml, HYP dose-dependently and significantly (P < 0.001) inhibited Sorghum bicolor seed radicle growth throughout 24-72 h compared to the negative control. Conclusion: Hyptis pectinata aerial part aqueous extract (HYP) possessed antioxidant and antiproliferative activities, hence providing...
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Posted by admin on Jan 11, 2018 in |
Diabetes mellitus (DM) is being one of the major causes of morbidity and mortality, which seriously threatens the health of human beings. Worldwide around 366 million people are found to be affected by diabetes. DM is a chronic condition associated with abnormally high levels of sugar (glucose) in the blood. Absence or insufficient production of insulin, or an inability of the body to properly use insulin causes diabetes. DM can be classified as either type 1 or type 2. Type 1 diabetes conditions or insulin-dependent, which treated by insulin therapy. Type 2 diabetes or non-insulin-dependent, which treated by oral hypoglycemic drugs. Conventional insulin and other oral anti-diabetic agents delivery associated with many drawbacks, the need for frequent monitoring of blood glucose, multiple insulin injections, and adjustment of insulin dosages, self-injection, and difficulties in using the vial and syringe technique are among the barriers to patient compliance. Most oral anti-diabetic drugs exhibit low oral bioavailability and need frequent dosing owing to short half-lives, resulting in poor patient compliance. Therefore, to...
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Posted by admin on Jan 28, 2017 in |
Loganin is an iridoid glycoside, a key phytoconstituent in Strychnos plants having a crucial role in the biosynthesis of indole, monoterpene alkaloids and other natural products. It is a bitter iridoid glycoside with numerous activities like anti-inflammatory, anti-oxidant, glucose-lowering and anti-diabetic nephropathy activities. The objective of this study was to develop a simple method for isolation of loganin from fruit pulp of Nux vomica and quantify by HPLC. The isolated compound was characterized by TLC, UV, IR, MS, and NMR spectral analysis and purity were confirmed by HPLC analysis. Quantification of loganin was done from fruit, seeds and pulp extract using HPLC. A simple solvent partitioning method was used for the extraction and isolation process. This isolation method developed involved easy solvent-solvent extraction technique followed by recrystallization to purify the...
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Posted by admin on Jan 28, 2017 in |
As per the appeal issued by World Health Organization (WHO) “save plants to save lives,” there is the need for a global movement for the conservation of medicinal plants and the revitalization of the native health traditions of local communities. Such health traditions based on medicinal plants are the result of centuries of observation, selection, and experimentation. They thus constitute an accumulated pool of medical wisdom, based on the dictum “an ounce of practice is worth tons of theory.” Unfortunately, such invaluable knowledge and experience are increasingly coming under the category of ‘dying wisdom.’ Throughout human history people have relied on natural products and plants, in particular, to promote and maintain good health and to fight sickness, pain, and disease. The past 200 years have witnessed not only an acceleration in the rate of extinction of plant and animal species but also the erosion of traditional knowledge related to the medicinal properties and uses of the plant and other natural products. India also had a rich tradition for primary...
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Posted by admin on Jan 28, 2017 in |
A study was conducted to assess root, leaves and flowers extracts of Cichorium intybus for their antimicrobial activities (against four bacterial and fungal strains), antioxidants activities (DPPH, ABTS H2O2 and reducing power assays) and cytotoxicities assays. The results indicate remarkable inhibition of plant extracts for the growth of all bacterial and fungal strains as well as appropriate MIC values were observed as compared to standard antibiotics used. The activities of these extracts against four assays like DPPH, H2O2, ABTS, and reducing powder was promising. Whereas the cytotoxic activity of extracts study against brine shrimp was reliable. The phytochemical analysis of the various parts of Cichorium intybus revealed that activities of these extracts might be due to the presence of various secondary metabolites like phenolics and flavonoids those were analyzed with higher quantities from these extracts. Therefore such a study could be useful for the development of new pharmaceuticals that could lead such compounds for the preparation of new medicines required for human and animals...
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