THE EFFECT OF CHLOROFORM EXTRACT OF GERMAN CHAMOMILE (MATRICARIA RECUTITA) ON ESCHERICHIA COLI INFECTED MICEAbstract
Emergence of new strains of Escherichia coli with the ability of resistance to a wide range of antibiotics necessitates the efforts to search alternatives for better treatments to deal with these infections. Chamomile is one of the most widely used herbs in the world. In this study, we investigated the potential therapeutic effects of chamomile chloroform extract on E. coli intraperitoneal (IP) infection of BALB/c mice. Female BALB/c with an average weight of 20 to 25 g, were divided into 8 groups. The lethal dose of the E. coli for mice has injected IP in test groups and treated with 5.25, 10.5 and 21 mg/mL of chamomile chloroform extract. The combined effect of the extract (50 µg/mg) with amikacin and amikacin alone were also examined. The highest number of survived mice at the dose of 10.5 mg/mL of extract and the highest death rate with 5.25 mg/mL of extract was observed. In the amikacin group, all infected animals were survived. The mortality rate in chamomile extract combination with amikacin is almost the same as amikacin alone. The significant lowest mortality rate was observed in some group which had been treated with a combination of extract and amikacin. The result of this study does not recommend in-vivo using of chloroform extracts of chamomile for E. coli infection via IP administration. In vivo chloroform extracts of chamomile, it seems not to have a strong antibacterial effect on E. coli IP infection of BALB/c mice.
M. Motamedifar, H. Sedigh*, D. Mansury and A. Rajabi
Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences Shiraz, Iran.
13 October 2013
24 November 2013
27 December 2013
01 January 2014