SERPYLLIFOLIA LEAVES EXTRACT ON STREPTOZOTOCIN-INDUCED DIABETIC RATSAbstract
The purpose of the study was to determine the leaves of Andrographis serpyllifolia for anti-diabetic and antihyperlipidemic effects in streptozotocin-induced diabetic rats. The aqueous, alcoholic and hydroalcoholic extracts of Andrographis serpyllifolia were tested for toxicity up to 4000 mg kg-1 as per OECD-425 guidelines. Anti-diabetic activity was assessed by oral glucose tolerance test and streptozotocin-induced model. In oral glucose tolerance test alcoholic and hydroalcoholic extracts exhibited greater activity compared to aqueous extract. Hence the alcoholic and hydroalcoholic extracts were further screened by the streptozotocin-induced model at 75, 150 and 300mg kg-1 for 15 days. The alcoholic and hydroalcoholic extracts effectively lowered serum glucose, triglycerides, cholesterol, low-density lipoproteins, very low-density lipoproteins, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, creatinine, and elevated high-density lipoprotein levels, body weight and liver glycogen levels. The alcoholic and hydroalcoholic extracts elicited dose-dependent effect, and the effect produced at the lower dose was not considerable. Hydroalcoholic extract (300 mg kg-1) elicited greater anti-diabetic and antihyperlipidemic activity compared to alcoholic extract (300 mg kg-1).
S. S. Gupta, L. Azmi, I. Shukla, L. Pal, P. K. Mohaptra and C. V. Rao *
Pharmacognosy and Ethnopharmacology Division, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India.
12 July 2016
19 August 2016
19 August 2016
31 August 2016