PHYTOCHEMICAL, IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF ARGYREIA PILOSA WIGHT & ARN. (WHOLE PLANT)Abstract
Background: The whole plant of Argyreia pilosa Wight & Arn. (Convolvulaceae) continuously to be utilized in indigenous Indian systems as a remedy for cough, quinsy and applied externally in case of itch, eczema and other skin troubles, antidiabetic, antiphlogistic, rheumatism and reduce burning sensation. Still, there were constrained phytochemical or biological studies on the whole plant of A. pilosa, as well as no studies which align using its conventional medicinal uses. Aim: This study aimed to determine the total tannin and flavonoids contents, antimicrobial and antioxidant activity of two extracts of the whole plant of A. pilosa and compare them to find out the better extract for upcoming studies. Materials and Methods: The whole plant material was subjected to cold maceration with ethyl acetate and methanol to obtain ethyl acetate extract (APEE) and methanolic extract (APME). Phytochemical constituents of these extracts were determined as per standard procedure. Total phenolics had been estimated by the Folin–Ciocalteu colorimetric method using tannic acid as standard. Total flavonoids had been estimated by aluminium chloride assay using quercetin as standard, and the antioxidant capacity was determined by the 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, hydrogen peroxide assay and reducing capacity. The antimicrobial activity was studied with the agar diffusion method, and minimal inhibitory concentrations (MIC) of these extracts were determined. Results: APEE has a better yield than APME. The phytochemical constituents present in both APEE and APME have revealed the presence of flavonoids, alkaloids, phenols, tannins, glycosides, amino acids and proteins except for steroids and acid compounds which are only present in APME. Total tannin content and total flavonoids content of APEE is more than in APME. Both REE and RME have antioxidant and antibacterial potency. Statistically, there is no significant difference between the antioxidant potency of APEE and APME. But APEE and APME are statistically different from each other in terms of their antibacterial strength, APEE being better than APME in this case.
D. S. N. B. K. Prasanth *, A. S. Rao and R. P. Yejella
Department of Pharmacy, JNTUK, Kakinada, Andhra Pradesh, India.
15 January 2017
22 March 2017
25 March 2017
01 April 2017