PHYTOCHEMICAL AND ANTIBACTERIAL POTENTIAL OF ACETONE LEAF EXTRACT OF ACACIA CONCINNA (WILLD.) DC.

The present study was aimed to find out the antimicrobial potential of acetone extract of Acacia concinna leaf against five different bacterial strains at 100 and 200mg/ml concentrations. The disc diffusion assay was performed to access the antimicrobial potential of the extract. The agar plate containing a bacterial culture was divided into 4 zones, and each zone was independently impregnated with four sterile filter paper discs (6 mm in diameter) containing (10 µl of each concentration) distill water (Negative control), ampicillin (Positive control); Acacia concinna leaf extract (100 mg/ml and 200 mg/ml as test). Zone of inhibition and MIC (minimum inhibitory concentration) for test drug was evaluated to access the antimicrobial potency. The phytochemical analysis shows the presence of alkaloids, tannins, and glycosides that might be responsible for the aimed biological activities. Out of five bacterial strains, the test drug exhibit zone of inhibition against Mycobacterium smegmatis and Bacillus subtilis in a concentration-dependent manner. Minimum inhibitory concentration (MIC) of test drug against Mycobacterium smegmatis and Bacillus subtilis, was found to be 2.5 (mg/ml) and 1.25 (mg/ml) respectively. The basis of above findings suggests the need for further future perspective works on the plant extract of Acacia concinna which might be very useful as it seems to be a potential source for arresting the growth and metabolic activities of strains like Mycobacterium smegmatis and Bacillus subtilis.