IN-VITRO HYALURONIDASE INHIBITION PROPERTIES OF ALOE CAMPERI, ALOE PERCRASSA AND SENNA SINGUEANA

In folk medicine, Aloe camperi, Aloe percrassa (Aloeceae), and Senna singueana L. (Fabaceae) are used in the management of infectious and inflammation-related disorders. The objective of this work was to evaluate the in-vitro hyaluronidase inhibition activities of extracts from these three medicinal plants. Various concentrations (10, 50, and 100 µg/ml) of leaf latex from the two Aloe sp and hydro alcoholic extract of S. singueana leaves were screened using hyaluronidase inhibition assay. The results showed that all the three extracts displayed concentration-dependent inhibitory activities with IC₅₀ values of 771.78, 664.47 and 630.83 μg/ml for A. camperi, A. Percrassa and S. singueana, respectively. Indomethacin, used as a standard drug in this study, showed IC₅₀ value of 27.95μg/ml. Hyaluronidase hydrolyzes hyaluronic acid in the extracellular matrix during tissue remodeling. Since, oligomers from hyaluronic acid degradation are associated with induction of inflammation and hyaluronidase activity up-regulation occurs in chronic inflammatory conditions, hyaluronidase inhibitors are suggested to have a beneficial role in prevention and treatment of inflammation-related disorders. Thus, the hyaluronidase enzyme inhibition activity of extracts from A. camperi, A. percrassa, and S. singueana could partially contribute to their traditional use against infectious and inflammatory related disorders.