IDENTIFICATION OF ENDEMIC CURCUMA ALBIFLORA THW. FROM CURCUMA SPECIES GROWN IN SRI LANKA BY DNA BARCODING METHOD
AbstractCurcuma is an important genus in traditional medicine. Five plants are reported in Sri Lanka; C. albiflora, C. aromatica, C. longa, C. zedoaria, and C. oligantha. Since the phylogenetic analysis of gene sequences and combining with complete genomic sequences helps to identify the genetic basis of plants, the current study was conducted. Standard CTAB method with little modifications was used for the extraction and purification. Extracted DNA was amplified using universal primers for matK genes in chloroplast genome by PCR (polymerase chain reaction). Sequenced fragments were analyzed and used for DNA barcoding. DNA barcodes {Accession Numbers: C. aromatica (GU180412), C. albiflora (KF 521885), C. longa (JQ409688), C. oligantha (JQ409715), C. zedoaria (JQ409703)} were used for analysis. C. albiflora appeared as a different group as per the Neighbor-Joining method and therefore, it can be identified as a new group. The matK gene of C. albiflora and other species showed, totally 222 variable sites. Therefore, the matK gene was an appropriate DNA barcode for identifying C. albiflora from remaining Curcuma plants grown in Sri Lanka.
Article Information
5
223-225
596
832
English
IJP
H. M. I. C. Herath, G. A. S. Premakumara and T. D. C. M. K. Wijayasiriwardene *
Industrial Technology Institute, Bauddaloka Mawatha, Colombo, Sri Lanka.
drchandima@iti.lk
19 November 2017
05 January 2018
13 February 2018
10.13040/IJPSR.0975-8232.IJP.5(4).223-25
01 April 2018
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