HPLC METHOD VALIDATION OF MOXIFLOXACIN
AbstractMoxifloxacin is a broad-spectrum antibiotic. Moxifloxacin is a 4th generation fluoroquinolone. Chemically it is 8-methoxy-fluoroquinolone. It acts by inhibiting DNA gyrase, a type topoisomerase II and topoisomerase IV. These are enzymes which are necessary for separation of bacterial DNA, so inhibits the replication of the cell. Its study was based on single-drug delivery, intravenous. The apparatus which have been used is a reversed-phase Li Christopher RP-18 with a column and a mobile phase comprising different solvents. Fluorescence detection was carried out at a higher wavelength of 290 nm and an emission wavelength of 550 nm. This method causes a short retention time (8.0 min) for moxifloxacin. The linear curves were obtained from 5-250 μg/L in plasma and from 0.1-2.5 μg/g of brain tissue. Limit of quantification (LOD) was 5 μg/L in plasma and 0.1 μg/g in brain tissue. The method causes the detection of systemic antimicrobial agents in plasma and CNS of infected mice by Listeria monocytogenes.
Article Information
3
763-768
688
1507
English
IJP
M. Hanif *, V. Chaurasiya , U. Farooq , S. H. Sarwer and S. Nawaz
Department of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan
phrvesh@gmail.com
28 April 2014
20 October 2014
15 November 2014
http://dx.doi.org/10.13040/IJPSR.0975-8232.IJP.1(12).763-68
01 December 2014
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