DETERMINATION OF TOTAL FLAVONOID CONTENT, TOTAL PHENOLIC CONTENT, GC-MS AND HPTLC ANALYSIS OF HYDRO ALCOHOLIC EXTRACT OF DRIED LEAVES OF PORTULACA GRANDIFLORA HOOK

To perform phytochemical screening, estimate total phenolics and total flavonoid content of hydro alcoholic extract of dried leaves of Portulaca grandiflora Hook. The shade- dried leaves of P. grandiflora (100 g) were extracted exhaustively by Soxhlet with hydro- alcohol. The conc. hydro-alcoholic extract were further studied to quantitative determination, GC-MS and HPTLC to identify and characterize the chemical compound present in the crude extract. The phenolic content present in hydro-alcoholic leaf extract was found to be 1.57µg/ml (µg of Gallic acid/mg of extract) and flavonoid content was found to be 2.73µg/ml (µg of Rutin/mg of extract). Phytochemical analysis of hydroalcoholic extract showed presence of major classes of phytochemicals. High performance – thin layer chromatography results revealed the mobile phase of ethyl acetate: formic acid: glacial acetic acid: water (10:0.5:0.5:1.3), the fluorescent band under (254 nmand366 nm) at Rf 0.21 was identified, and the marker chemical rutin was measured. Gas chromatography – mass spectroscopy results showed the presence of 27 phytoconstituents including phenolic acids, ketones, flavonoids, carbohydrates, and hydrocarbons. The results of this study support the traditional usage of P. grandiflora, a plant that contains a number of known and unidentified bioactive chemicals that may be utilised as a powerful source against a number of ailments.