COMPARATION BETWEEN TRADEMARK AND ANALYTICAL STANDARD CHLORPYRIFOS ADMINISTRATION ON BUTYRYLCHOLINESTERASE AND GLUCOSE LEVELS IN RATS

Indonesia is one of the largest agricultural countries in the world. In 2022, approximately 88.89% of the workforce was employed in the agricultural sector. One of the most important agricultural practices is the use of pesticides, which are essential for pest control. Organophosphates are widely used pesticides, and chlorpyrifos is one of the most commonly applied organophosphate insecticides. However, chlorpyrifos exposure may cause adverse health effects, including poisoning, organ damage, mortality, and insulin resistance. Butyrylcholinesterase (BChE) activity and serum glucose levels are important biomarkers for evaluating chlorpyrifos toxicity, as exposure to chlorpyrifos can decrease BChE activity and increase serum glucose levels. Chlorpyrifos is available in two forms, analytical standard chlorpyrifos (Pestanal) and trademark formulations such as Dursban 200 EC. However, studies comparing the effects of trademark and analytical standard chlorpyrifos on BChE and serum glucose levels are still limited. This study aimed to compare BChE activity and serum glucose levels between control and treatment groups, as well as between Pestanal and Dursban treatments. Rats were orally administered Pestanal or Dursban at a dose of 5 mg/kg body weight for 28 days. Serum BChE activity and glucose levels were measured and analyzed using an independent t-test with SPSS software. The results showed that serum BChE activity differed significantly compared to the control group and between the Pestanal and Dursban groups. Serum glucose levels were significantly higher in the treatment groups compared to the control group. However, no significant difference was observed between the Pestanal and Dursban groups.