IN-VITRO CYTOTOXICITY OF ASSAY OF LEAVES OF PORTULACA QUADRIFIDA USING BRINE SHRIMP ASSAYAbstract
Portulaca quadrifida Linn. (Portulacaceae) is commonly known as chicken weed in English. In traditional system it is used to treat rheumatism and gynaecological diseases, urinary tract infections, worm diseases, dysentry and dermatitis. To assess cytotoxic potential of hydroalcoholic extract o f P. quadrifida leaves using brine shrimp assay. Potassium dichromate was considered as standard reference drug. The concentrations of 10, 100, 1000 μg/ml were used in the experiment. The mature shrimps were taken and it was treated with the extract and the standard in the respective concentrations. LC50 value was calculated for both standard and extract and the probit analysis were made by using SPSS software. From the probit analysis the cytotoxicity of the extract was determined by comparing it with the standard. Graph was plotted for both the standard and the extract, the extract was found to be non- toxic in nature.
R. T. Kumaran * and N. Naidu
Department of Pharmacognosy, Faculty of Pharmacy, Sri Ramachandra Medical College and Research Institute, Porur, Chennai, Tamil Nadu, India.
27 March, 2018
12 June, 2018
17 June, 2018
01 July, 2018