ANTISICKLING PROPERTIES OF THREE MEDICINAL PLANTS AND THEIR COMBINATIONAbstract
Background: Sickle cell anemia continues to be prevalent in the African population of which Nigeria alone has epidemiology of 25%, and only a few official medications are available, necessitating this current study. Methods: Cnestis ferruginea Vahl ex DC., Connaraceae, Alcornea laxiflora (Benth.) Pax & K. Hoffman Euphorbiaceae, and Spathodea campanulata Beauv., Bignoniaceae, which have various uses ethnomedicinally in Nigeria were extracted using a cold method of extraction with absolute methanol. The abilities of the extracts individually and in combination, to inhibit or reverse sickling of erythrocytes in-vitro at low oxygen tensions according to the modified methodology of Dean and Schechter, 1978 were examined. Results: While C. ferruginea leaf gave 94.6% sickling reversal activity at 4 mg/ml, S. campanulata gave reversal activity of 89.6% at 4 mg/ml, and A. laxiflora leaf gave the highest sickling inhibitory activity of 98.8% at 8 mg/ml. The combination of A. laxiflora leaf and C. ferruginea leaves when extracted together at ratio 1:2 gave an inhibition activity of 91.7% at 4mg/ml and 93.5% inhibition at ratio 2:1 at 8 mg/ml, these values were statistically significant at P˂0.05. Conclusion: This study shows that some of these plants were more active when tested individually than when in any combinations.
V. O. Bamimore * and A. A. Elujoba
Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
10 August 2018
25 September 2018
30 September 2018
01 October 2018