ANTIFERTILITY EFFECT OF CAESALPINIA BONDUCELLA (L.) FLEMING IN MALE WISTAR RATHTML Full Text
ANTIFERTILITY EFFECT OF CAESALPINIA BONDUCELLA (L.) FLEMING IN MALE WISTAR RAT
Priyanka Meerwal and Gyan Chand Jain
Department of Zoology, University of Rajasthan, Jaipur - 302004, Rajasthan, India
ABSTRACT: To evaluate the possible antifertility effect of 50% ethanolic extract of C. bonducella seeds in male rats. Adult, healthy, male Wistar rats were randomly selected and divided into three groups each having 7 rats. Group I rats were treated with vehicle only and served as control. While group II and Group III rats were orally treated with C. bonducella extract at 200 mg and 400 mg/kg body weight/day, respectively for 60 days. The result of the present study indicated that there was significant decrease in the relative weights of testes and accessory sex glands in treated rats. The density, motility and viability of spermatozoa obtained from cauda epididymis were significantly (P<0.01 at 200 mg and P<0.001 at 400 mg) diminished in both treated groups as compared to control group. The serum testosterone level also showed significant (P<0.05 at 200 mg and P<0.01 at 400 mg) decline. The fertility rate and litter size decreased significantly in extract treated rats. Extract treatment also induced an alteration in biochemical milieu of the genital organs. The histopathological study of the tests showed dose dependent degenerative and atrophic changes. The seminiferous tubules and Leydig cells were shrinked. The number of germ cells and spermatozoa in seminiferous tubules were depleted. Further, histological study of epididymis also showed degenerative changes in the epithelium and only few spermatozoa in the lumen of tubules. In conclusion, 50% ethanolic extract of C. bonducella seeds showed significant antiandrogenic, antispermatogenic effect and consequently decline in fertility of male Wistar rats
Cauda epididymis, C .bonducella, testis, testosterone, spermatozoa
INTRODUCTION: Population explosion is one of the biggest problems all over the World with its inevitable consequences on all development, especially employment, education, housing, health care, sanitation and environment. The control of fertility in the sense of its limitation especially in developing centuries is the most important and urgent need today. During the last few decades a large number of plants have been screened and searched thoroughly for their possible antifertility effects in males in order to develop male contraceptive agent of plant origin 1, 2.
Caesalpinia bonducella L. Fleming (Family-Fabaceae/Caesalpiniaceae) Commonly known as “Katikaranja”, is a prickly shrub widely distributed all over the world specially in hotter parts of India, Srilanka and Myanmar. It is a very valuable medicinal plant, utilized in traditional system of medicine for the treatment of a large number of ailments. The seeds have a bitter taste, are valued in traditional medicine as a tonic, antiperiodic and recommended for treating fever, asthma and colic.
They are used in Ayurvedic system of medicine for treating diarrhoea, intestinal worms, hydrocoele, liver and spleen diseases, diabetes, leprosy and piles, They are ingredient of several Ayurvedic preparations. The leaves and bark are used as an emmenagogue, antihelminthic and febrifuge 3. The seeds are traditionally used in the fertility regulation in rural India 4, 5, 6. Sugalis of Andhra Pradesh use seed powder mixed with Palmyra palm sugar orally for promoting sterility in men 7.
Experimental studies have shown that C. bonducella seeds possess many pharmacological activites, like adaptogenic 8, antioxidant and antitumor 9, antidiabetic 10, immunomodulatory 11, anti-inflammatory 12, antimicrobial 13, antiulcer 14 and antifilarial 15. Various pharmacological studies of C. bonducella have been reviewed by many workers 16, 17, 18. Ethanolic extract of C. bonducella seeds has also been reported to possess anti-implantation and abortifacient potential in female rats 19. Salunka et al 20 reported a decrease in ovarian weight and duration of esterous cycle in female rats by virtue of antiestrogenic activity of alcoholic extract of C. bonducella seeds. Phytochemical analysis of C. bonducella seeds revealed the presence of alkaloids, glycosides, saponins, tannis, triterpenoids 16, 18, 21.
Literature survey reveals that no systematic approach has been made to study the antifertility activity of seeds of this plant in males despite its traditional use in inhibition of male fertility. Therefore, the present study was undertaken to study the antifertility effect of 50% ethanolic extract of C. bonducella seeds in male Wistar rats.
MATERIALS AND METHODS:
Plant extract: Seeds of C. bonducella were collected from local area of Smriti Van, Jaipur, Rajasthan, India. The seeds were identified and authenticated at the Herbarium, Department of Botany, University of Rajasthan, Jaipur under the specimen voucher no. (RUBL 211342). The seeds were dried, coarsely powdered and soxhleted with 50% ethanol at 55˚-60˚ C for 35 h. The extract was filtered and concentrated under low temperature and pressure to obtain a dry viscous dark brownish mass. A suspension of the extract was prepared in sterile distilled water. The required extract dose was administered orally with a glass syringe fitted with a feeding needle.
Colony bred, adult healthy male Wistar rats weighing 170-200 g, were used in the present study. The rats were housed in polypropylene cages and maintained under controlled temperature (23±1ºC) and lighting conditions (12 h light/dark cycle). Standard laboratory chow (Aashirwad Food Industries, Chandigarh, India) and water was provided ad libitum. The experimental study was approved by the Ethical Committee of the Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur. The Indian National Science Academy (2000) guidelines were followed for maintenance and use of the experimental animals.
Experimental design: Adult, healthy, colony bred Wistar male rats of proven fertility were divided randomly into three groups of 7 rats each.
Group A: Control rats received 0.5 ml/day of the vehicle, i.e., distilled water.
Group B: Rats were treated with 50% ethanolic extract of C. bonducella seeds at a dose of 200 mg/kg body weight /day, orally, suspended in distilled water for 60 days.
Group C: Rats were treated with 50% ethanolic extract of C. bonducella seeds at a dose of 400 mg/kg body weight/day, orally, suspended in distilled water for 60 days.
Twenty-four hours after their last dose, the overnight fasted rats were weighted and sacrificed under light ether anaesthesia.
Body and organ weights:
Initial and final body weights of each rat were recorded. The testes, epididymides, vas deferens, seminal vesicle, ventral prostate were dissected out, freed from adherent tissues and blood and weighed to the nearest milligram.
Testis, epididymis and seminal vesicle were frozen at -20ºc for the biochemical estimations. Testis and epididymis were analysed for total protein 22, glycogen 23, sialic acid 24, and total cholesterol 25. Fructose in seminal vesicle was also estimated according to method of Mann 26.
Testosterone hormone: Blood samples were collected from the cardiac puncture and allowed to clot at room temperature. Serum was separated by centrifugation at 2000 rpm for 20 min at 4ºC and stored at -20°C for hormone assays.The serum testosterone was measured with the help of chemiluminescence immuno assay.
To determine sperm motility and sperm count, one hundred milligram of the cauda epididymis was minced with a sharp razor blade in 2 ml of physiological saline (0.9% Nacl 37ºc). For sperm motility, one drop of evenly mixed sample was applied to a glass slide under a cover glass. The percent motility was determined by counting both motile and immotile spermatozoa in different area 27. Sperm counts were made by routine procedure using Neubauer counting chamber, and expressed as million/ml of suspension 28. Sperm vitality was assessed by nigrosin-eosin staining method.
One drop of the 1% aqueous solution of eosin-y and 10% aqueous solution of nigrosin was placed in a microcentrifuge tube. A drop of well mixed sperm sample was added to it and mixed thoroughly. The mixture was dropped on a glass slide and observed under light microscope at 400X magnification. The percentage of live (without stain) and dead (red) cells were determined by at least counting 200 cells 29.
Extract treated male rats were paired with normal parous females (male: female ratio, 1:2) at 17:00 h daily after 55 days of treatment. The successful mating was confirmed in the forthcoming morning from 56 to 61 days by presence of vaginal plug and spermatozoa in the vaginal smear. The inseminated females were separated and allowed to deliver at term. The numbers of pups delivered were recorded 27.
For the histological examination, the testis and cauda epididymis were fixed in Bouin’s fixative, washed in water, dehydrated in graded ethanol series, cleared in xylene and embedded in paraffin wax. Section were cut at 5 micrometer and stained with hematoxylin and eosin. The stained slides were observed under light microscope for histopathological changes. Mean seminiferous tubular diameter were determined with the help of light microscope equipped with ocular micrometer calibrated with stage micrometer at X 100.Diameter of Leydig cell nucleus was also measured with the help ocular micrometer at X 1000 and expressed as mean value.
Data are expressed as Mean ± SEM. Statistical difference in the mean was analysed using one way ANOVA followed by Turkey’s test. P <0.05 was considered as statistically significant.
Effect on body and organ weight:
Although there was significant gain in the body weight of all the control (P<0.001) and experimental rats (P<0.01) when compared to their respective initial body weight but, the control rats registered more gain in body weight as compared to extract treated rats. Animals exposed to 50% ethanolic extract of C. bonducella seeds at 200 and 400 mg/kg body weight/day doses showed a significant dose dependent decrease in the relative weights of testes (P<0.05, P<0.001), epididymides (P<0.05, P<0.001), vas deferens (P<0.05, P<0.01), seminal vesicle (P<0.05, P<0.01) and ventral prostate (P<0.05, P<0.01) respectively, when compared with control rats (Table 1).
The total protein and sialic acid contents of the testis and epididymis were decreased significantly in rats of both treated groups [Group B (P<0.05) and Group C (P<0.01)]. However, the concentrations of glycogen and cholesterol were significantly elevated in both testis and epididymis of extract treated rats. A significant dose dependent decline in seminal vesicle fructose content was also recorded in extract treated rats as compared to control rats. (Table 2).
A significant decline in serum testosterone level was observed in rats of both treated groups. [Group B (P<0.05) and Group C (P<0.01)] as compared to control rats (Table 3).
Sperm analysis and fertility test:
The sperm counts and sperm motility were significantly decreased in group B (P<0.01) and group C (P<0.001) animals when compared to control animals. The percentage of viable sperms also reduced significantly in both Group B (P<0.05) and Group C (P<0.001) when compared to Group A (control).The fertility rate and number of litter size were also declined significantly in extract treated rats. (Table 3, 4).
TABLE 1: EFFECT OF C. BONDUCELLA SEED EXTRACT TREATMENT ON THE BODY AND ORGAN WEIGHTS OF MALE RATS
|Initial||Final||(mg / 100 g b. wt.)|
|Group- BC.bonducella (200 mg/kg b. wt.)||178.57±3.57||197.86±4.48**(+10.80%)||1163.74±25.89 a||503.00±15.38a||143.16±4.25 a||511.95±8.69 a||243.29±4.70 a|
|Group- CC.bonducella (400 mg/kg b. wt.)||175.71±3.17||191.43±2.61**(+8.94%)||1094.71±21.51c||472.83±21.08 c||139.30±4.01 b||495.13±10.98 b||222.76±9.31 b|
Levels of significance: Values represent mean ± SEM of 7 Animals
**- P<0.01; ***- P<0.001 when compared with initial body weight
a- P<0.05; b- P<0.01; c- P<0.001 when compared with control
TABLE 2: EFFECT OF C. BONDUCELLA SEED EXTRACT TREATMENT ON VARIOUS TISSUE BIOCHEMICAL PARAMETERS OF MALE RATS
|Treatments||Total protein(mg/g)||Glycogen(mg/g)||Sialic acid(mg/g)||Total cholesterol(mg/g)||Fructose(mg/g)|
|Group-AControl (vehicle)||209 ±6.72||226.14±7.71||3.46±0.11||3.24±0.09||5.85±0.09||6.02±0.10||6.19±0.28||6.65±0.10||5.54±0.17|
|Group-B C.bonducella(200 mg/kg b.wt.)||187.71±5.69a||201.14±4.77a||4.29±0.25a||4.11±0.24a||5.39±0.11a||5.52±0.10a||7.30±0.23b||7.16±0.11a||4.86±0.19 a|
|Group -CC. bonducella (400 mg/kg b. wt.)||177.57±3.59b||192.29±6.03b||4.69±0.27b||4.25±0.23b||5.24±0.10b||5.39±0.09b||7.92±0.11c||7.36±0.10b||4.39±0.15c|
Levels of significance: Values represent mean ± SEM of 7 Animals
a- P<0.05; b- P<0.01; c- P<0.001 when compared with control
TABLE 3: EFFECT OF C. BONDUCELLA SEED EXTRACT TREATMENT ON SERUM TESTOSTERONE, SPERM ANALYSIS AND MORPHOMETRIC PARAMETERS OF MALE RAT
|Treatments||Serum testosterone(ng/ml)||Sperm count
(Million / ml)
|Sperm viability(%)||Seminiferous tubular diameter(µ m)||Leydig cell nuclear diameter(µ m)|
|Group-B C.bonducella (200 mg/kg b.wt.)||6.38±0.16a||42.87±2.58 b||48.62±7.18b||56.77±4.63 a||228.57±11.91 a||6.71±0.18 a|
|Group -CC.bonducella (400 mg/kg b. wt.)||6.10±0.28b||27.80±1.56c||33.89±3.53c||38.14±6.36c||213.57±10.53b||6.14±0.26b|
Levels of significance: Values represent mean ± SEM of 7 Animals
a- P<0.05; b- P<0.01; c- P<0.001 when compared with control
TABLE 4: EFFECT OF C. BONDUCELLA SEED EXTRACT TREATMENT ON FERTILITY RATE AND LITTER SIZE
|Treatments||No. of males||No. of females||No of pregnant females||No. of foetuses delivered||Fertility%|
|Group-BC. bonducella (200 mg/kg b. wt.)||7||14||9/14||5.14±0.91b||64.29|
|Group-CC. bonducella (400 mg/kg b. wt.)||7||14||5/14||3.29±0.87c||35.71|
Levels of significance:
a- P<0.05; b- P<0.01; c- P<0.001 when compared with control
Histopathological findings in the testis and epididymis:
The photomicrographs of the testis of control rats showed normal structure of seminiferous tubules surrounded by vascularised dense fibrous connective tissue with prominent Leydig cells. The germinal epithelium in seminiferous tubules exhibited normal shape, size and characteristic arrangement of all successive germ cell types. The lumen was filled with large number of healthy spermatozoa (Fig.1) The histoarchitecture of the testis treated with two different dose of C. bonducella (200 and 400 mg/kg body weight/day) showed dose dependent degenerative and atrophic changes. The seminiferous tubules were shrinked, the numbers of germ cells were reduced and the lumen contains sloughed debris and few spermatozoa. The Leydig cells also showed atrophic changes with reduced size of the nuclei (Fig. 2, 3) Histological study of cauda epididymis of control rat showed epididymal tubules lined with tall columnar cells bearing large number of stereocilia and lumen is full of spermatozoa (Fig-4) The cauda epididymis of extract treated rats showed dose dependent degenerative changes. The epithelial cells lining the tubules were reduced in height and showed degenerative changes. The lumen contains sperm debris and only few spermatozoa. The intertubular space was also increased (Fig. 5, 6).
FIG.1: PHOTOMICROGRAPH OF THE CROSS SECTION OF TESTIS OF CONTROL RAT SHOWING NORMAL SEMINIFEROUS TUBULES WITH ALL TYPE OF SPERMATOGENIC ELEMENTS AND A LARGE NUMBER OF SPERMATOZOA IN THE LUMEN (H. E. X 200).
FIGURE: 2. PHOTOMICROGRAPH OF THE CROSS SECTION OF TESTIS OF C.BONDUCELLA EXTRACT (200 mg/kg b. wt. /DAY) TREATED RAT SHOWING MILD DEGENERATIVE CHANGES IN SEMINIFEROUS TUBULES (H. E. X 200).
FIG. 3: PHOTOMICROGRAPH OF THE CROSS SECTION OF TESTIS OF C.BONDUCELLA EXTRACT (400 mg/ kg b. wt. /DAY) TREATED RAT SHOWING MARKED SHRINKAGE OF SEMINIFEROUS TUBULES, DEGENERATIVE CHANGES IN GERM CELLS WITH DISRUPTION OF SPERMATOGENESIS LEYDIG CELLS ALSO SHOWING ATROPHIC CHANGESAND REDUCED DIAMETER OF NUCLEI (H. E. X 200).
FIG. 4: PHOTOMICROGRAPH OF THE CROSS SECTION OF CAUDA EPIDIDYMIS OF CONTROL RAT SHOWING NORMAL TUBULES LINED WITH TALL COLUMNAR CELLS. THE LUMEN CONTAINS LARGE NUMBER OF SPERMATOZOA (H. E. X 200).
FIG.5: PHOTOMICROGRAPH OF THE CROSS SECTION OF CAUDA EPIDIDYMIS OF C. BONDUCELLA (200 mg/ kg b. wt. /DAY) TREATED RAT SHOWING DEGENERATIVE CHANGES AND REDUCED EPITHELIAL CELL HEIGHT (H.E.X 200).
FIG. 6: PHOTOMICROGRAPH OF THE CROSS SECTION OF CAUDA EPIDIDYMIS OF C .BONDUCELLA EXTRACT (400 mg/kg b. wt. /DAY) TREATED RAT SHOWING A SEVERELY REDUCED EPITHELIAL CELL HEIGHT AND INCREASE IN INTERTUBULAR STROMA. LUMEN CONTAINS SPERM DEBRIS AND FEW SPERMATOZOA (H. E. X 200).
DISCUSSION: The findings of the present study showed that the 50% ethanolic seed extract of C. bonducella induced significant antifertility effects in male rats. The assessment of the weights of reproductive organs is one of the most sensitive parameter for the study of the effect of any substance on the male reproductive system 30. In the present study, oral administration of 50% ehanolic extract of C. bonducella seeds for 60 days brought a significant decline in the relative weight of testes, epididymides, vas-deferens, seminal vesicle and ventral prostate. The weight of the testis largely depends on the mass of developing germ cells, spermatids and spermatozoa 31, 32. The reduction in testicular weight observed in the present study might be due to a reduction in testicular size, spermatogenic arrest and inhibition of steroid biosynthesis by the Leydig cells. The decrease of relative weight of epididymis may be due to decline in sperm count or reduced bioavailability of testosterone. It is well recognized that testosterone is crucial for the maintenance of structural integrity and functional activity of accessory sex organs 33, 34. A significant decline in the relative weights of seminal vesicle and ventral prostate could be due to reduced growth and absence of secretion in the lumen as a consequence of androgen depletion 35.
Analysis of sperm parameters viz, sperm density, motility and viability are indices of spermatogenesis and sperm maturation and provide evidence about the effect on fertility and fecundity 36. Sperm analysis in C. bonducella extract treated rats exhibited a significant dose depended decline in the sperm count, motility and number of viable spermatozoa. Sperm cell count is considered the most sensitive test for spermatogenesis and it is highly correlated with fertility 37. The diminution of epididymal sperm count in extract treated rats may be due to adverse impact of the extract on spermatogenesis. The epididymis plays an important role in sperm development, maturation, transport and storage where it depends on the luminal environment of epididymis 38. The decline in percentage of motile and viable spermatozoa in extract treated rats in the present study might have resulted from the alteration in the epididymal milieu due to androgen deficiency and also due to toxic effects on cellular levels. 39, 40.
The loss of motility of spermatozoa might also be due to an adverse effect on oxidative/energy metabolism which might disrupts the energy supply for sperm motion 41. These results are in agreement with earlier reports where extract of C. bonducella seeds has been reported to cause decline in sperm density 42 and to increase morphological abnormalties in spermatozoa of rats 43.
Treatment with 50% ethanolic extract of C. bonducella seeds brought a significant decline in fertility rate (%) and litter size. This decline in fertility and litter size may be attributed to deterioration of sperm quality and density 44, 45, since the inadequate concentration, sluggish motile or immotile spermatozoa could not penetrate the cervical mucus and thus failed to fertilize the ova 46, 47.
Testosterone is the main circulating androgen synthesized and secreted by Leydig cells under the influence of pituitary luteinizing hormone (LH) 48. In the present study, a significant dose dependent decline in the levels of serum testosterone was observed in rats treated with C. bonducella extract. Histological observation of testis of extract treated rats also supports these findings, as we observed shrinkage and atrophic changes in the Leydig cells. The increased testicular cholesterol content in extract treated rats also support adverse impact on steroidogenesis. Decline in serum testosterone might be due to inhibitory effect of the plant extract on pituitary LH secretion or through direct action on the Leydig cells resulting in inhibition of steroidogenic activites. C.bonducella seed extract has been reported to possess antiestrogenic effect in female rats due to presence of phytoestrogens 20, which might interfere in estrogen negative feedback by binding to estrogen receptors in the anterior pituitary or hypothalamus and thereby reduce secretion of the gonadotropin releasing hormone (GnRH), follicle stimulating hormone (FSH) and luteinizing hormone (LH) resulting in the decline of testosterone biosynthesis and secretion in Leydig cells.
It has been reported that protein level is directly correlated with the secretory activity of the testis and epididymis which is dependent on androgen level 49. Oral treatment of rats with C. bonducella extract also induce biochemical alteration in the milieu of testis and epididymis. The present study indicates a significant reduction in the concentration of protein in testis and epididymis. Protein biosynthesis is a key factor for testicular development and spermatogenesis. Sertoli cells synthesize and secrete a variety of proteins required during spermatogenesis 50. The decline in protein content in testis might be due to disturbances in cellular metabolism, lack of normal level and activity of testicular androgens and decrease in the number of spermatozoa in the testis 51. The decrease in the protein concentration in epididymis possibly affects the internal milieu of the organ and consequently maturation of spermatozoa. Similar decrease in protein contents of the testis and epididymis have been reported due to treatment of many plant extracts in rats possibly due to antiandrogenic effect 52, 53.
Increase in glycogen content in both testis and epididymis indicates under utilization of glycogen either due to inhibitory effect on glycogen phosphorylase or glycolytic enzymes 54. Sialic acid is an important constituent of glycoprotein and glycolipids and is essential for the maintenance of the structural integrity of sperm membrane and sperm maturation 55. The reduction in the sialic acid concentration in testis and epididymis may be correlated with impairment of androgen secretion along with necrotic and atrophic changes in principal cells of the epididymis 53, 56.
Cholesterol is an essential component of mammalian cell membrane and also acts as precursor for steroid hormone biosynthesis in gonads. Significant increase of testicular cholesterol concentration in the extract treated rats reflects reduced conversion of cholesterol to androgen or the arrest of steroidogenesis in Leydig cells leading to accumulation of cholesterol in testes 54, 51. Increased in cholesterol concentration in epididymis might result in disturbances in sperm maturation and fertilizing ability of spermatozoa. Cholesterol homeostasis regulation is crucial for post testicular sperm maturation and imbalanced cholesterol level may affect these post-testicular events 57. Fructose is an important source of energy for motility of spermatozoa. A significant reduction in fructose content of seminal vesicle reflects impairment of secretory function of the gland attributed to androgen depletion 58. A similar decrease in seminal vesicle fructose level mediated by antiandrogenic effect of various plant extracts have been reported earlier by many workers 54, 40.
Histological examination of the testis of 50% ethanolic extract of C. bonducella seeds showed significant dose dependent degenerative, atrophic changes and impairment of spermatogenesis. The seminiferous tubules were shrinked and the number of post-meotic germ cells and spermatozoa in the lumen were significantly reduced. The tiered arrangement of the germ cells was distributed. The Leydig cells were shrunken and showed atrophic changes. These observed histopathological changes in testis possibly occurred as a consequence of decline in serum testosterone which is essential for normal spermatogenesis as well as for the maintenance of structural morphology and normal physiology of seminiferous tubules 59. The reduction in the number of post-meotic germ cells in extract treated rats again indicate diminished testosterone level, as testosterone is essential for post-meotic division during spermatogenesis 60.
According to Sharpe 61, the male reproductive system possesses estrogenic receptor α (ER-α) and β (ER-β). Alcoholic extract of C. bonducella seeds has been reported to induce antiestrogenic effect due to presence of estrogenic phytoconstituents. 20 Hence, an estrogenic overload may occur as a consequence of the binding of constituents present in the extract to the estrogenic receptors. Many chemicals which bind to estrogen receptors on male genital organs have shown similar degenerative changes and impairment of spermatogenesis by virtue of decline in serum testosterone 31, 62.
Estrogen is likely to affect Leydig cell function through at least two mechanisms, including suppression of LH release by negative feedback and a direct effect on the steroidogenesis 63, 64. Our results are in agreement with previous studies where plant extracts rich in phytoestrogens have shown similar degenerative changes and impairment of spermatogenesis in testes 65, 66.
Administration of 50% ehanolic extract of C. bonducella seeds in rats also caused significant dose dependent degenerative changes in cauda epididymis. The epithelial cells lining the epididymal tubules were reduced in height, showed degenerative changes and bear short stereocilia. The lumen shared the presence of sperm debris and scanty spermatozoa. The intertubular stroma was also increased. The observed changes in cauda epididymis of extract treated rats may be correlated with deficit androgen supply. These results are in agreement with previous finding where similar type of degenerative changes and decline of spermatozoa in lumen have been reported as a consequence of decrease of androgen supply in rats treated with various plant extracts 67, 68, 69, 70.
CONCLUSIONS: From the results of the present study it may be concluded that phytoconstituents present in the 50% ethanol extract of C. bonducella seeds by virtue of their individual or synergistic activity induced significant antispermatogenic and antiandrogenic effect leading to decline of fertility in male Wistar rats. Further studies at higher doses are in progress.
ACKNOWLEDGMENTS: The authors are thankful to Head of the department and coordinator, CAS for providing necessary facilities and also to University Grant Commission for awarding Rajiv Gandhi National Fellowship to Priyanka Meerwal.
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How to cite this article:
Meerwal P and Jain GC: Antifertility Effect of Caesalpinia Bonducella (L.) Fleming in Male Wistar Rat. Int J Pharmacognosy 2016; 3(6): 265-75:.doi link: http://dx.doi.org/10.13040/IJPSR.0975-8232.IJP.3(6).265-75.
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