SUBSTANTIATION OF USING CHLORELLA GENUS MICROALGAE AS A RAW MATERIAL FOR PREPARATION OF CHEMOPREVENTIVE SUBSTANCESAbstract
The purpose of this study was to substantiate the use of suspension of Chlorella genus microalgae as the most valuable raw material for the preparation of carotene and chlorophyll а. The method is proposed for ultrasonication of Chlorella vulgaris microalgae suspension to increase the availability of biologically active substances and improve the microbiological performance of the prepared product. Before and after ultrasonication of samples, we determined the content of chlorophylls (a, b, c) and carotenoids using the spectrophotometric method, the level of total bacterial contamination using the method of limiting dilutions on MacConkey agar and microscopic study of microalgae and bacteria cell viability. It has been established that the ultrasonication method at wave amplitude 16 µm allows to transfer pigments into the water phase, inhibit the viability of bacteria and obtain homogenization of the Chlorella vulgaris microalgae suspension with preservation of carotenoids and green chlorophyll а. Thus, the combined use of ultrasonication and parallel plating on MacConkey agar seems to be promising given preparation and quality control of axenic concentrate of Chlorella vulgaris microalgae, which can be used for preparation of chemopreventive pharmaceuticals.
E. V. Тretiakova *, P. G. Beliaeva and A. I. Saralov
Department of Pharmaceutical Technology, Perm State Pharmaceutical Academy - 2, Polevaya Str., Perm, 614990, Russia.
31 October 2018
22 November 2018
27 November 2018
01 December 2018