STANDARDIZATION OF MARKETED AMRITARISHTA- A HERBAL FORMULATIONAbstract
The present study aims to standardize Amritarishta based upon chromatographic and spectral studies. The spectral data and HPTLC fingerprint of ethanolic extract of Amritarishta could be used as a valuable analytical tool in the routine standardization of Amritarishta to check the batch to bath variation. Fraction, I and II of ethanolic extract of Amritarishta and standard luteolin and apigenin, were compared by TLC, HPTLC, and HPLC analysis to evaluate the presence of luteolin and apigenin is employing toluene: ethyl acetate: glacial acetic acid (5:4:1 v/v/v), as a mobile phase respectively. The Rf values (0.64) and (0.81) for luteolin and apigenin in both sample and reference standard were found comparable under UV light at 366 nm respectively. The High-Performance Thin Layer Chromatography method developed for quantization was simple, accurate and specific. The present standardization provides a specific and accurate tool to develop qualifications for identity, transparency, and reproducibility of biomarkers luteolin and apigenin in a liquid formulation.
K. A. Wadkar *, M. S. Kondawar and S. G. Lokapure
Department of Pharmacognosy, Appasaheb Birnale College of Pharmacy, South Shivaji Nagar, Sangli, Maharashtra, India
08 August 2016
15 September 2016
27 September 2016
30 September 2016