SIMPLE METHOD OF ISOLATION OF LOGANIN FROM NUX VOMICA FRUITS AND ITS QUANTIFICATION BY HPLC
AbstractLoganin is an iridoid glycoside, a key phytoconstituent in Strychnos plants having a crucial role in the biosynthesis of indole, monoterpene alkaloids and other natural products. It is a bitter iridoid glycoside with numerous activities like anti-inflammatory, anti-oxidant, glucose-lowering and anti-diabetic nephropathy activities. The objective of this study was to develop a simple method for isolation of loganin from fruit pulp of Nux vomica and quantify by HPLC. The isolated compound was characterized by TLC, UV, IR, MS, and NMR spectral analysis and purity were confirmed by HPLC analysis. Quantification of loganin was done from fruit, seeds and pulp extract using HPLC. A simple solvent partitioning method was used for the extraction and isolation process. This isolation method developed involved easy solvent-solvent extraction technique followed by recrystallization to purify the compound.
Article Information
6
39-42
527
1037
English
IJP
S. P. Patil * and K. S. Laddha
Medicinal and Natural Product Research Laboratory, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Matunga, Mumbai, Maharashtra, India,
p.patilsapna@gmail.com
16 November 2016
17 December 2016
26 December 2016
10.13040/IJPSR.0975-8232.IJP.4(1).39-42
01 January 2017