PHYTOCHEMICAL ANALYSIS AND INVESTIGATION OF ANTI-INFLAMMATORY AND ANTI-ULCER ACTIVITY OF TERMINALIA BELLIRICA LEAVES EXTRACTAbstract
The present study was undertaken to provide the rationale for the use of Terminalia bellirica as an anti-inflammatory agent using carrageenan-induced inflammation and antiulcer agent using ethanol-acid induced gastric mucosal injury model in the Swiss albino rats. The anti-inflammatory activity of the extract was evaluated using carrageenan (1% w/v) induced inflammation model at doses of 50, 120 and 300 mg/kg, p.o while using ibuprofen (20 mg/kg, p.o) as the standard drug. On the other hand, the antiulcer activity of methanolic extract of T. bellirica leaves at the doses of 100, 250 and 500 mg/kg, p.o. were examined against ethanol-acid induced gastric mucosal injury in the Swiss albino rats – keeping omeprazole (20 mg/kg, p.o.) as the reference. The rats were dissected, and their stomachs were macroscopically examined to identify hemorrhagic lesions in the glandular mucosa. Further, the histopathological changes of paws and stomachs were analyzed. T. bellirica significantly (P<0.01) decreased carrageenan-induced paw edema; it exhibited a reduction of 50.00%, 55.88% and 61.76% at doses of 50, 120 and 300 mg/kg respectively. The methanolic extract also disclosed a good protective effect against ethanol-acid induced gastric mucosal injury in the rats. Oral administration of the extract`s doses (100, 250 and 500 mg/kg) demonstrated a significant (P<0.05) reduction in the ethanol- acid-induced gastric erosion in all the experimental groups when compared to the control. These findings were further supported by the histological study. The study clearly shows that methanolic extract of T. bellirica leaf possesses potent anti-inflammatory activity and promotes ulcer protection as ascertained by regeneration of mucosal layer and substantial prevention of the formation of hemorrhage and edema.
S. Akter, T. Begum*, R. Begum, T. S. Tonny, M. Yasmin, S. Shifa, F. Afroze and Faruquzzaman
Department of Pharmacy, Primeasia University, Banani, Dhaka, Bangladesh.
25 January 2019
20 February 2019
23 February 2019
28 February 2019