PHARMACOGNOSTIC STUDIES OF STEM TUBER ON BRASSICA OLERACEA VAR. GONGYLODESAbstract
The crude extracts from stem tuber and leaf of Brassica oleracea var. gongylodes in different solvents, were subjected to physicochemical, fluorescence analysis, phytochemical and antimicrobial study. The microscopic analysis revealed that B. oleracea showed stone cells and calcium oxalate prisms. The ash value was high 7.88 % in B. Oleracea. The fluorescent analysis at 366 nm of B. oleracea under ultraviolet light revealed that powder extracted with alcohol showed pale blue colored fluorescence, with water showed yellow color, 0.1 N Sodium hydroxide showed pale yellow and dilute hydrochloric acid had greenish yellow. The extraction value with different solvents exhibited that B. oleracea maximum extractive value with chloroform followed by benzene, ethanol and petroleum ether. The phytochemical screening of the various extract of B. oleracea revealed the presence of alkaloids, flavonoids, resins, saponins, and tannins. Anti-bacterial screening in B. oleracea using stem revealed that the ethanol solvent showed maximum inhibition against gram-positive bacteria (Staphylococcus aureus) and chloroform solvent showed maximum inhibition in the case of gram-negative bacteria (Serratia marcescence).