IN-VIVO STUDY OF ANTILITHIATIC ACTIVITY ON THE FRUITS EXTRACTS OF COCCINIA INDICA (WIGHT & ARN.) ETHYLENE GLYCOL INDUCED LITHIATIC IN RATSAbstract
Aim of the Study: This study aimed to investigate the effect of ethanolic extract of fruits Coccinia indica Wight & Arn. (Cucurbitaceae) on urolithiasis in rats. Materials & methods: Thirty-six male rats were randomly divided into six groups (n = 6). Ethylene glycol (EG) 0.75% in drinking water were fed to all groups (Groups II-VI) except normal control (Group I) rats for 15-28th days to induce urolithiasis. Group III-VI rats were treated with ethanolic extract Coccinia indica of at doses 100, 200, 300 mg/kg, respectively, for 28 days. Positive control (Group II) rats were treated with EG alone. Group, I rats was administered drinking water and distilled water (6 μl/g) by gavage. After 15-28th days, blood samples were collected and analyzed for serum concentrations of calcium, Inorganic phosphorus, Blood urea, and creatinine. The kidneys were removed and sectioned for histopathological examination. The data were presented as mean ± standard error of the mean and analyzed using one-way analysis of variance P<0.05 was considered statistically significant. Results: In the present in-vivo study, renal stone inducing treatment to male rats resulted in hyperoxaluria. Ethanolic extract of Coccinia indica (at 100 and 200 mg/kg) exhibited a dose-dependent significant antilithiatic activity on treatment. The extract dose of 100 mg/kg also caused reduction of calcium, oxalates, phosphorus, and creatinine in blood serum level the results were found statistically significant. The antilithiatic effect of ethanol extract at was found effective than the reference standard. Histopathology of the kidneys in Groups V and VI revealed less tissue damage and were almost similar to Group I rats.
M. Kumar *, S. Alok, S. K. Jain and A. Verma
Department of Pharmacognosy, Institute of Pharmacy Bundelkhand University, Jhansi, Uttar Pradesh, India.
17 October 2013
24 November 2013
15 December 2013
01 January 2014