HPLC METHOD VALIDATION OF MOXIFLOXACINAbstract
Moxifloxacin is a broad-spectrum antibiotic. Moxifloxacin is a 4th generation fluoroquinolone. Chemically it is 8-methoxy-fluoroquinolone. It acts by inhibiting DNA gyrase, a type topoisomerase II and topoisomerase IV. These are enzymes which are necessary for separation of bacterial DNA, so inhibits the replication of the cell. Its study was based on single-drug delivery, intravenous. The apparatus which have been used is a reversed-phase Li Christopher RP-18 with a column and a mobile phase comprising different solvents. Fluorescence detection was carried out at a higher wavelength of 290 nm and an emission wavelength of 550 nm. This method causes a short retention time (8.0 min) for moxifloxacin. The linear curves were obtained from 5-250 μg/L in plasma and from 0.1-2.5 μg/g of brain tissue. Limit of quantification (LOD) was 5 μg/L in plasma and 0.1 μg/g in brain tissue. The method causes the detection of systemic antimicrobial agents in plasma and CNS of infected mice by Listeria monocytogenes.
M. Hanif *, V. Chaurasiya , U. Farooq , S. H. Sarwer and S. Nawaz
Department of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan
28 April 2014
20 October 2014
15 November 2014
01 December 2014