EFFECTS OF AQUEOUS METHANOLIC LEAF EXTRACTS OF INDIGENOUS PLANTS CLERODENDRUM COLEBROOKIANUM WALP AND CENTELLA ASIATICA LINN. ON CARBON TETRACHLORIDE INDUCED CARDIOTOXIC WISTER ALBINO RATSAbstract
Two indigenous plants Clerodendrum colebrookianum walp and Centella asiatica Linn. were selected to determine its cardioprotective activity on male Wister albino rats. Empirical research was designed to evaluate the cardioprotective activity of the leaves of both with special reference to its putative protective role. Aqueous methanolic extracts of both the plants were prepared by continuous hot percolation method. Male albino rats of Wistar strain (250-300 g) were used for the study. Animals were divided into 10 groups of six animals each. Both pretreatment and post-treatment study was done for 16 days at two different doses of 300 mg/kg body weight and 700 mg/kg body weight, respectively. Carbon tetrachloride was used as a cardiotoxic agent. The in-vivo biological studies on serum and tissues of male Wister rats at the doses of 300 mg and 700 mg/kg body weight respectively was carried out taking silymarin as standard. The methanolic extract of both improved the cholesterol level along with signiﬁcant improvement of SGPT (Serum glutamate pyruvate transaminase), SGOT (Serum glutamate oxaloacetate transaminase), ALP (Alkaline phosphatase) and total protein in respect to silymarin group. The test extract at higher dose was found to signiﬁcantly reverse the elevated marker enzymes, i.e. SGOT, SGPT, ALP indicating its cardioprotective role. The higher dose extracts were also found to have a pronounced effect on oxidative stress parameters such as GSH and catalase on CCl4 induced rats. Followed by heart tissue separation and homogenization was done in KCl (10 mM) phosphate buffer (1.15%) with ethylene-diamine-tetraacetic acid (EDTA: pH 7.4) and centrifuged at 12000 rpm for 20 min. The supernatant was used for the measurement of malondialdehyde (MDA). Lipid peroxidation was estimated in terms of thiobarbituric acid reactive species (TBARS) using MDA as standard by the method of Buege and Aust 1978. The amount of MDA was calculated using a molar extinction coefficient of 1.56 × 105 M-1 cm-1 and expressed as nmoles of MDA formed per mg wet weight of tissue. For both pretreatment study and post-treatment study MDA concentration was maximum for the toxic control group and minimum for the control group. For the extracts of C. colebrookianum and C. asiatica at both the dose level 300 mg/kg body weight and 700mg/kg body weight is showing significant (p< 0.01) reduction in MDA production. For C. colebrookianum MDA production is less on a higher dose, and for C. asiatica at a low dose, only MDA production is less. It signifies both the plant extracts are having potent antioxidant activities, and it is evidenced from previous literature also.