APPLICATION OF MOLECULAR BEACON BASED BIOSENSOR AGAINST rs699 SNP IN HYPERTENSIVE AND NON- HYPERTENSIVE PUNJABI POPULATIONAbstract
Molecular beacons have shown their topnotch potential in a variety of basic research, biomedical detection and clinical diagnosis. Their excellent selectivity, sensitivity, detection without separation have made them widely accepted tool for nucleic acid analysis. MBs present high-throughput screening of SNPs. The use of SNPs in the detection of genetic disorders is facilitated by the recent discovery of more than 4 million SNPs in the human genome. The objective of this study was to explore the association of rs699 SNP with essential hypertension in Punjabi Population. MB based biosensor developed by Verma et al., in 2016 was chosen for analysis. These hypertensive subjects tested positive against hypertensive biosensor (against allele C) were associated with essential hypertension. C4072T or rs699 polymorphism was genotyped in 50 hypertensive and 50 normotensive subjects. The p-value for the C allele in hypertensive patients was 0.71, which concludes susceptibility to hypertension in this population when the CC genotype is present. The implementation was cross-validated by applying the same samples to Normotensive Biosensor (against T allele). The presence of fluorescence with hypertensive biosensor confirms the presence of a mutation in hypertensive patients or patients that are clinically naive but can develop hypertension in the forthcoming due to hypertensive allele presence. Hence it can help in the future pharmacogenetic based treatment of patients.