ANTIPSEUDOMONAL ACTIVITY OF ARTEMISIA QUETTENSIS ESSENTIAL OIL AND ITS SYNERGY WITH IMIPENEMAbstract
Over the past two decades, to increase the antimicrobial spectrum of antibiotics, effective compounds of plants have been used synergically with antibiotics. The plants of genus Artemisia (Asteraceae) have been conventionally used for prevention and medication of several ailments. Due to the significance of antimicrobial activity of Artemisia species, this study aimed to evaluate the effect of essential oil of Artemisia quettensis individually, and in combination with imipenem, to inhibit the growth of Pseudomonas aeruginosa. The singular activity of essential oil and activity, when combined with an antibiotic, was hence elucidated. The essential oil was obtained through hydrodistillation from aerial parts of the plant and analysis using GC and GC-MS. The most dominant components were homoadamantane (9.38%), camphor (7.91%) and eugenol (10.46%). The oil and antibiotic showed high antibacterial activity against Pseudomonas aeruginosa with minimal inhibitory concentration (MIC) 0.5 µl/mL and 16 µg/mL, respectively. The main purpose of this research is the synergistic effect, the oil and antibiotic showed MIC 0.2 µl/mL and antibiotics 4 µg/mL, respectively. This study showed that Artemisia quettensis oil has significant antibacterial activity against Pseudomonas aeruginosa infections.
E. Saffari, M. A. N. Khalili * and J. F. Mehrabadi
Department of Biochemistry and Biophysics, Education and Research Center of Science and Biotechnology, Malek Ashtar University of Technology, Tehran, Iran.
31 October 2018
22 November 2018
27 November 2018
01 December 2018