ANTIPROLIFERATIVE ACTIVITY OF THREE WILD GROWING SPECIES IN TUNISIA: NICOTIANA GLAUCA, ARTEMISIA CAMPESTRIS AND ASTRAGALUS GOMBOAbstract
Tunisian flora contains a number of wild plants with diverse therapeutic uses. As a contribution to the efforts to select natural sources of antitumor compounds, we investigated in this study the Antiproliferative activity of several extracts of the Tunisian species: Astragalus gombo, Nicotiana glauca and Artemisia campestris. Dried aerial part (A. gombo and A. campestris) and leaves (N. glauca) were extracted with ethyle acetate, dichloromethane, and methanol. In vitro antiproliferative activity of the extracts was tested against the human solid tumor cell lines: HBL-100, T-47D, and WiDr. Tests were performed using the sulforhodamine B (SRB) assay. All extracts of A. campestris and A. gombo were active against all tested cell lines, with GI50 values between 12 and 93 µg/ml. Dichloromethane extract of A. campestris was the most active extract with GI50 of 12 µg/ml against HBL-100 cell line. This kind of cell seems to be the most sensitive regarding all tested extracts. Our results showed that A. gombo, A. campestris and N. glauca are a promising source of antitumor natural compounds. More detailed studies should be conducted especially for most active extracts.
H. Teyeb*, W. Douki and J. M. Padrón
Biochemistry and Toxicology Laboratory, University Hospital of Monastir, University of Monastir, Monastir 5000, Tunisia.
09 May, 2017
08 July, 2017
11 July, 2017
01 September, 2017