ANTIFUNGAL STUDY OF CHEMICAL CONSTITUENTS ISOLATED FROM PIPER CALDENSE C. D.C.Abstract
An intensive phytochemical study of the two parts of the Piper caldense plant brings the isolation of secondary metabolites, generated by shikimate and the mevalonate pathways. Using chromatographic techniques, the ethanolic extract of leaves afforded two pheophytyns, the pheophytin (1), 151-hydroxy-pophyrinlactone a (2), a mixture of the β-sitosterol and stigmasterol steroids (3-4). Four derivatives of benzoic acid (5-8) and the trilinoein (9) were isolated from the fruits. The spectroscopic methods such as 1H and 13C Nuclear Magnetic Resonance (NMR) and electrospray mass spectrometry (ESI-MS) were used for structural characterization. The compounds were screened for their in-vitro antifungal activity against seven Candida strains. The control used in the antifungal assays was nystatin. In general, three compounds revealed fungal sensitivity, especially, the prenylated benzoic acid derivative (6) which accomplished the lowest minimum inhibitory concentration (MIC).
G. A. T. Silva, E. B. V. de Sousa, J. U. G. Jardim, R. C. Montes *, B. F. Lira, J. Í. V. Costa, E. O. Lima, J. M. B. Filho and M. C. O. Chaves
Department of Pharmaceutical Sciences, Federal University of Paraíba, Universidade Federal da Paraíba, João Pessoa, Brazil.
15 May 2018
07 June 2018
13 June 2018
01 August 2018