AMINO ACID ANALYSIS USING ION- EXCHANGE CHROMATOGRAPHY: A REVIEWAbstract
Amino acids are biologically active substances, and a number of them are essential for living beings. Amino acids are found in living cells as well as in body fluids of higher animals, in amounts, which vary according to the tissue and particular amino acid. Amino acid analysis refers to the methodology used to determine the amino acid composition or content of proteins, peptides, and other pharmaceutical preparations. Proteins and peptides are macromolecules consisting of covalently bonded amino acid residues organized as a linear polymer. The sequence of the amino acids in a protein or peptide determines the properties of the molecule. Proteins are considered large molecules that commonly exist as folded structures with a specific conformation, while peptides are smaller and may consist of only a few amino acids. Amino acid analysis can be used to quantify protein and peptides, to determine the identity of proteins or peptides based on their amino acid composition, to support protein and peptide structure analysis, to evaluate fragmentation strategies for peptide mapping, and to detect atypical amino acids that might be present in a protein or peptide. It is necessary to hydrolyze a protein/peptide to its amino acid constituents before amino acid analysis. The perfect method for the determination of the amino acid composition of pure protein feeds or biological fluids is still the ion exchange column chromatography (IEC).
C. Singh *, C. S. Sharma and P. R. Kamble
Department of Quality Assurance, Bhupal Nobels’ College of Pharmacy, Udaipur, Rajasthan, India.
19 April 2014
18 August 2014
28 August 2014
01 September 2014